Title :

Comparison of cellular and transcriptomic effects between electronic cigarette vapor and cigarette smoke in human bronchial epithelial cells

Author(s) :

Anthérieu, Sébastien [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Garat, Anne [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Beauval, Nicolas [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Soyez, Mélissa [Auteur]
Allorge, Delphine [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Garcon, Guillaume [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Lo Guidice, Jean-Marc [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]

Journal title :

Toxicology in Vitro

Abbreviated title :

Toxicology in Vitro

Pages :

417-425

Publisher :

Elsevier BV

Publication date :

2017-12

ISSN :

0887-2333

HAL domain(s) :

Sciences du Vivant [q-bio]/Médecine humaine et pathologie

English abstract : [en]

The use of electronic cigarette (e-cig) can be considered as an alternative to smoking. However, due to a lack of thorough toxicological studies, absolute safety of these products cannot be guaranteed. The aim of this in ...
Show more >The use of electronic cigarette (e-cig) can be considered as an alternative to smoking. However, due to a lack of thorough toxicological studies, absolute safety of these products cannot be guaranteed. The aim of this in vitro work was to investigate the potential toxicity of e-vapors generated by a smoking machine in human bronchial epithelial BEAS-2B cells cultured at air-liquid interface, in comparison to cigarette smoke (CS). Although CS decreased strongly cell viability from 48min exposure, e-vapors induced no cytotoxicity up to 288min exposure. Moreover, oxidative stress was evidenced only after exposure to CS, with a decrease secretion of GRO-ɑ from 8min and of IL-8 and MCP-1 after 48min exposure. Only a low increase of IL-6 secretion was measured in cells exposed to e-vapors. Finally, transcriptomic data of exposed cells indicated that a large number of genes were deregulated in response to CS, especially genes involved in important biological functions as oxidative stress and cell death, while e-vapors elicited very discrete modulation. These results strongly suggest a lower toxicity of e-vapors compared to CS in the BEAS-2B cell line and constitute a baseline for further experimental studies with a larger spectrum of e-liquids and e-cig models.Show less >

Peer reviewed article :

Oui

Audience :

Non spécifiée

Administrative institution(s) :

Université de Lille
CHU Lille
Institut Pasteur de Lille

Collections :

• IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483

Submission date :

2020-10-19T15:11:00Z
2021-03-25T12:46:28Z
2021-03-25T12:49:12Z