FRET-Based Enzyme Activity Reporter: ...
Document type :
Partie d'ouvrage: Chapitre
DOI :
Permalink :
Title :
FRET-Based Enzyme Activity Reporter: Practical Hints for Kinases as Indicators of Virulence
Author(s) :
Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Kasprowicz, Angelina [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Trinel, Dave [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bodart, Jean-Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Kasprowicz, Angelina [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Trinel, Dave [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bodart, Jean-Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Book title :
Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis
Publisher :
InTech
Publication date :
2018-03-21
ISBN :
978-953-51-3916-4
English keyword(s) :
genetically encoded biosensor
KAR
fret
MAPK
ERK
KAR
fret
MAPK
ERK
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes ...
Show more >Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes of these reporters, which are now within the arsenal of biosensors, were devoted to the detection and characterization of the activity of the cAMP-Protein kinase A pathway. Based on the principle of this biosensor, other FRET-based kinase activity reporters emerged. Here, the choice of the kinase to be monitored, the artifacts that might be met, and the flexibility and amenability of the FRET-based kinase activity reporters both for high-throughput analysis and dissection of protein kinase functions are discussed.Show less >
Show more >Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes of these reporters, which are now within the arsenal of biosensors, were devoted to the detection and characterization of the activity of the cAMP-Protein kinase A pathway. Based on the principle of this biosensor, other FRET-based kinase activity reporters emerged. Here, the choice of the kinase to be monitored, the artifacts that might be met, and the flexibility and amenability of the FRET-based kinase activity reporters both for high-throughput analysis and dissection of protein kinase functions are discussed.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Régulation des signaux de division
Submission date :
2020-11-12T15:41:10Z
2020-11-16T13:38:57Z
2021-01-07T10:58:37Z
2020-11-16T13:38:57Z
2021-01-07T10:58:37Z
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