Preliminary results on 5-ALA PDT fractionation on a preclinical rodent model

Mordon, Serge; Dupont, Clément; Betrouni, N.; Reyns, N.; Vermandel, M.; Leroy, H.A.; Quidet, M.; Leroux, B.

Type de document :

Autre communication scientifique (congrès sans actes - poster - séminaire...): Communication dans un congrès avec actes

DOI :

10.1016/j.pdpdt.2017.01.169

Titre :

Preliminary results on 5-ALA PDT fractionation on a preclinical rodent model

Auteur(s) :

Mordon, Serge [Auteur]

Thérapies Assistées par Lasers et Immunothérapies pour l'Oncologie - U 1189 [OncoThAI]
Dupont, Clément [Auteur]
SNECMA Villaroche [Moissy-Cramayel]
Betrouni, N. [Auteur]
Thérapies Assistées par Lasers et Immunothérapies pour l'Oncologie - U 1189 [OncoThAI]
Reyns, N. [Auteur]
Thérapies Assistées par Lasers et Immunothérapies pour l'Oncologie - U 1189 [OncoThAI]
Vermandel, M. [Auteur]
Thérapies Assistées par Lasers et Immunothérapies pour l'Oncologie - U 1189 [OncoThAI]
Leroy, H.A. [Auteur]
Quidet, M. [Auteur]
Leroux, B. [Auteur]
Centre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]

Titre de la manifestation scientifique :

IPA

Ville :

NANCY

Pays :

France

Date de début de la manifestation scientifique :

2016-10-24

Date de publication :

2017-03

Discipline(s) HAL :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Cancer

Résumé en anglais : [en]

Glioblastoma Multiform (GBM) is a high-grade brain tumour with local recurrence and poor outcome. Photodynamic therapy to treat glioblastoma has been recently reported to improve overall survival. However, controlled study ...
Lire la suite >Glioblastoma Multiform (GBM) is a high-grade brain tumour with local recurrence and poor outcome. Photodynamic therapy to treat glioblastoma has been recently reported to improve overall survival. However, controlled study to evidence treatment efficacy is still expected and treatment delivery has to be optimized. Several parameters might be optimized such as fluence rate, dose escalation or fractionation of light delivery. Specially, fractionation of light delivery is expected to enhance treatment efficiency by restoring tissue oxygenation. Thus, we have evaluated light fractionation on a preclinical rodent model harbouring grafted GBM. Thirty-nine " Nude " rats were grafted with human U87 cells. Cells were stereotactically grafted in rat brain. After the presence and the size of the tumour were assessed by microMRI, PDT was delivered interstitially. Light was delivered through an optical fibre introduced into the tumour after PS precursor intake (5-ALA). The rats were randomized in three groups: (1) without illumination, (2) with 2-steps light fractionation and (3) with 5-steps light fraction-ation. Brain immunohistology and microMRI images were achieved to evaluate the treatment effects. On diffusion MRI, elevated diffusion values in tumour centre among treated animals were observed, especially for the 5-steps fractionation group. Perfusion decreased around the treatment site, especially in the 5-steps fractionation group. Histology confirmed our MRI findings, with a more extensive necrosis and apopto-sis associated with a rarefiedangiogenic network in the treatment area, after 5-steps fractionation PDT. However, more surrounding oedema and neovascularization in the peripheral ring after 5-steps fractionation PDT were also noticed. Finally we observed that interstitial PDT induced more tumour necrosis and apoptosis when applying 5-steps fractionation. However , this scheme also led to significant peripheral oedema and neovascularization. Diffusion and perfusion were able to predict the histological lesions. Nevertheless, apoptosis cells death remains the preferred effect in order to decrease potential side effects. We hypothesize a lower fluence rate with the same light fractionation scheme might increase apoptosis while decreasing necrosis and oedema. Preclinical study is still on going to verify this hypothesis. http://dx.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

Thérapies Lasers Assistées par l'Image pour l'Oncologie (ONCO-THAI) - U1189

Source :

Harvested from HAL

Fichiers

https://hal.archives-ouvertes.fr/hal-01715241/file/Preliminary%20results%20on%205-ALA%20PDT%20fractionation%20on%20a%20preclinical%20rodent%20model.pdf
Accès libre
Accéder au document

Preliminary%20results%20on%205-ALA%20PDT%20fractionation%20on%20a%20preclinical%20rodent%20model.pdf
Accès libre
Accéder au document

Preliminary results on 5-ALA PDT fractionation ... BibTeX CSV Excel RIS

Fichiers

Preliminary results on 5-ALA PDT fractionation ...

BibTeX

CSV

Excel

RIS