Titre :

Towards two-photon excited endogenous fluorescence lifetime imaging microendoscopy

Auteur(s) :

Hage, C.H. [Auteur]
XLIM [XLIM]
Leclerc, P. [Auteur]
XLIM [XLIM]
Brevier, J. [Auteur]
XLIM [XLIM]
Fabert, M. [Auteur]
XLIM [XLIM]
Nézet, C. Le [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Kudlinski, A. [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Héliot, L. [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Louradour, D F. [Auteur]
XLIM [XLIM]

Titre de la revue :

Biomedical optics express

Pagination :

921 - 930

Éditeur :

Optical Society of America - OSA Publishing

Date de publication :

2017

ISSN :

2156-7085

Discipline(s) HAL :

Sciences de l'ingénieur [physics]/Optique / photonique

Résumé en anglais : [en]

In situ fluorescence lifetime imaging microscopy (FLIM) in an endoscopic configuration of the endogenous biomarker nicotinamide adenine dinucleotide (NADH) has a great potential for malignant tissue diagnosis. Moreover, ...
Lire la suite >In situ fluorescence lifetime imaging microscopy (FLIM) in an endoscopic configuration of the endogenous biomarker nicotinamide adenine dinucleotide (NADH) has a great potential for malignant tissue diagnosis. Moreover, two-photon nonlinear excitation provides intrinsic optical sectioning along with enhanced imaging depth. We demonstrate, for the first time to our knowledge, nonlinear endogenous FLIM in a fibered microscope with proximal detection, applied to NADH in cultured cells, as a first step to a nonlinear endomicroscope, using a double-clad microstructured fiber with convenient fiber length (> 3 m) and excitation pulse duration (≈50 fs). Fluorescence photons are collected by the fiber inner cladding and we show that its contribution to the impulse response function (IRF), which originates from its intermodal and chromatic dispersions, is small (< 600 ps) and stable for lengths up to 8 m and allows for short lifetime measurements. We use the phasor representation as a quick visualization tool adapted to the endoscopy speed requirements.Lire moins >

Langue :

Anglais

Vulgarisation :

Non

Projet ANR :

Regulation et rôles des kinases ERK1&2 (Extracelular Regulated Kinase 1&2) and Plk1 (Polo-like kinase 1) dans la progression G2/mitose

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL