Titre :

The Three-dimensional Study of Chromosomes and Upstream Binding Factor-immunolabeled Nucleolar Organizer Regions Demonstrates Their Nonrandom Spatial Arrangement during Mitosis

Auteur(s) :

Klein, Christophe [Auteur]
Cheutin, Thierry [Auteur]
Institut de génétique humaine [IGH]
Rothblum, Lawrence [Auteur]
Kaplan, Hervé [Auteur]
Beorchia, Adrien [Auteur]
Lucas, Laurent [Auteur]
Centre de Recherche en Sciences et Technologies de l'Information et de la Communication - EA 3804 [CRESTIC]
Héliot, Laurent [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Ploton, Dominique [Auteur]
O'Donohue, Marie-Françoise [Auteur]

Titre de la revue :

Molecular Biology of the Cell

Pagination :

3147-3159

Éditeur :

American Society for Cell Biology

Date de publication :

1998-11

Discipline(s) HAL :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biologie cellulaire

Résumé en anglais : [en]

The volumic rearrangement of both chromosomes and immunolabeled upstream binding factor in entire well-preserved mitotic cells was studied by confocal microscopy. By using high-quality three-dimensional visualization and ...
Lire la suite >The volumic rearrangement of both chromosomes and immunolabeled upstream binding factor in entire well-preserved mitotic cells was studied by confocal microscopy. By using high-quality three-dimensional visualization and tomography, it was possible to investigate interactively the volumic organization of chromosome sets and to focus on their internal characteristics. More particularly, this study demonstrates the nonrandom positioning of metaphase chromosomes bearing nucleolar organizer regions as revealed by their positive upstream binding factor immunolabeling. During the complex morphogenesis of the progeny nuclei from anaphase to late telophase, the equal partitioning of the nucleolar organizer regions is demonstrated by quantification, and their typical nonrandom central positioning within the chromosome sets is revealed.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL