A microbiota-generated bile salt induces biofilm formation in Clostridium difficile

Dubois, Thomas; Tremblay, Yannick D. N.; Hamiot, Audrey; Martin-Verstraete, Isabelle; Deschamps, Julien; Monot, Marc; Briandet, Romain; Dupuy, Bruno

Document type :

Article dans une revue scientifique

DOI :

10.1038/s41522-019-0087-4

Permalink :

http://hdl.handle.net/20.500.12210/36954

Title :

A microbiota-generated bile salt induces biofilm formation in Clostridium difficile

Author(s) :

Dubois, Thomas [Auteur]

Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes [PBA (U-Pasteur_6)]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Tremblay, Yannick D. N. [Auteur]
Hamiot, Audrey [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Pathogénèse des Bactéries Anaérobies / Pathogenesis of Bacterial Anaerobes [PBA (U-Pasteur_6)]
Martin-Verstraete, Isabelle [Auteur]
Deschamps, Julien [Auteur]
Monot, Marc [Auteur]
Briandet, Romain [Auteur]
Dupuy, Bruno [Auteur]

Journal title :

npj Biofilms and Microbiomes

Volume number :

Publisher :

Springer Science and Business Media LLC

Publication date :

2019-05-09

ISSN :

2055-5008

English keyword(s) :

Bacteriology
Biofilms
Pathogens

HAL domain(s) :

Sciences du Vivant [q-bio]/Ingénierie des aliments

English abstract : [en]

Clostridium difficile is a major cause of nosocomial infections. Bacterial persistence in the gut is responsible for infection relapse; sporulation and other unidentified mechanisms contribute to this process. Intestinal ...
Show more >Clostridium difficile is a major cause of nosocomial infections. Bacterial persistence in the gut is responsible for infection relapse; sporulation and other unidentified mechanisms contribute to this process. Intestinal bile salts cholate and deoxycholate stimulate spore germination, while deoxycholate kills vegetative cells. Here, we report that sub-lethal concentrations of deoxycholate stimulate biofilm formation, which protects C. difficile from antimicrobial compounds. The biofilm matrix is composed of extracellular DNA and proteinaceous factors that promote biofilm stability. Transcriptomic analysis indicates that deoxycholate induces metabolic pathways and cell envelope reorganization, and represses toxin and spore production. In support of the transcriptomic analysis, we show that global metabolic regulators and an uncharacterized lipoprotein contribute to deoxycholateinduced biofilm formation. Finally, Clostridium scindens enhances biofilm formation of C. difficile by converting cholate into deoxycholate. Together, our results suggest that deoxycholate is an intestinal signal that induces C. difficile persistence and may increase the risk of relapse.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Submission date :

2021-01-20T11:33:55Z

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Except where otherwise noted, this item's license is described as Attribution 3.0 United States

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