Effects of Ferrocenyl 4-(Imino)-1,4-Dihy ...
Document type :
Article dans une revue scientifique
PMID :
Permalink :
Title :
Effects of Ferrocenyl 4-(Imino)-1,4-Dihydro-quinolines on Prophase I - Arrested Oocytes: Survival and Hormonal-Induced M-Phase Entry.
Author(s) :
Marchand, Guillaume [Auteur]
Wambang, Nathalie [Auteur]
Pellegrini, Sylvain [Auteur]
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Molinaro, Caroline [Auteur]
Martoriati, Alain [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bousquet, Till [Auteur]
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Markey, Angel [Auteur]
Lescuyer-Rousseau, Arlette [Auteur]
Bodart, Jean-Francois [Auteur]
Cailliau, Katia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Pelinski, Lydie [Auteur]
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Marin, Matthieu [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Wambang, Nathalie [Auteur]
Pellegrini, Sylvain [Auteur]
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Molinaro, Caroline [Auteur]
Martoriati, Alain [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bousquet, Till [Auteur]
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Markey, Angel [Auteur]
Lescuyer-Rousseau, Arlette [Auteur]
Bodart, Jean-Francois [Auteur]
Cailliau, Katia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Pelinski, Lydie [Auteur]
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Marin, Matthieu [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Journal title :
International Journal of Molecular Sciences
Abbreviated title :
Int J Mol Sci
Volume number :
21
Publication date :
2020-04-26
ISSN :
1422-0067
English keyword(s) :
Xenopus laevis
cell death
ferrocenyldihydroquinoline
metaphase II spindle
oocyte
cell death
ferrocenyldihydroquinoline
metaphase II spindle
oocyte
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
oocytes were used as cellular and molecular sentinels to assess the effects of a new class of organometallic compounds called ferrocenyl dihydroquinolines that have been developed as potential anti-cancer agents. One ...
Show more >oocytes were used as cellular and molecular sentinels to assess the effects of a new class of organometallic compounds called ferrocenyl dihydroquinolines that have been developed as potential anti-cancer agents. One ferrocenyl dihydroquinoline compound exerted deleterious effects on oocyte survival after 48 h of incubation at 100 μM. Two ferrocenyl dihydroquinoline compounds had an inhibitory effect on the resumption of progesterone induced oocyte meiosis, compared to controls without ferrocenyl groups. In these inhibited oocytes, no MPF (Cdk1/cyclin B) activity was detected by western blot analysis as shown by the lack of phosphorylation of histone H3. The dephosphorylation of the inhibitory Y15 residue of Cdk1 occurred but cyclin B was degraded. Moreover, two apoptotic death markers, the active caspase 3 and the phosphorylated histone H2, were detected. Only 7-chloro-1-ferrocenylmethyl-4-(phenylylimino)-1,4-dihydroquinoline () did not show any toxicity and allowed the assembly of a histologically normal metaphase II meiotic spindle while inhibiting the proliferation of cancer cell lines with a low IC, suggesting that this compound appears suitable as an antimitotic agent.Show less >
Show more >oocytes were used as cellular and molecular sentinels to assess the effects of a new class of organometallic compounds called ferrocenyl dihydroquinolines that have been developed as potential anti-cancer agents. One ferrocenyl dihydroquinoline compound exerted deleterious effects on oocyte survival after 48 h of incubation at 100 μM. Two ferrocenyl dihydroquinoline compounds had an inhibitory effect on the resumption of progesterone induced oocyte meiosis, compared to controls without ferrocenyl groups. In these inhibited oocytes, no MPF (Cdk1/cyclin B) activity was detected by western blot analysis as shown by the lack of phosphorylation of histone H3. The dephosphorylation of the inhibitory Y15 residue of Cdk1 occurred but cyclin B was degraded. Moreover, two apoptotic death markers, the active caspase 3 and the phosphorylated histone H2, were detected. Only 7-chloro-1-ferrocenylmethyl-4-(phenylylimino)-1,4-dihydroquinoline () did not show any toxicity and allowed the assembly of a histologically normal metaphase II meiotic spindle while inhibiting the proliferation of cancer cell lines with a low IC, suggesting that this compound appears suitable as an antimitotic agent.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Régulation des signaux de division
Submission date :
2021-01-26T13:17:42Z
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