Title :

Expression, localization, and concentration of a-kinase anchor protein 4 (akap4) and its precursor (proakap4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa

Author(s) :

Blommaert, D. [Auteur]
Sergeant, N. [Auteur]
Delehedde, M. [Auteur]
Jouy, N. [Auteur]
Mitchell, V. [Auteur]
Franck, T. [Auteur]
Donnay, I. [Auteur]
Lejeune, J.-P. [Auteur]
Serteyn, D. [Auteur]

Journal title :

Theriogenology

Abbreviated title :

Theriogenology

Volume number :

131

Pages :

52-60

Publication date :

2019-03-21

ISSN :

1879-3231

Keyword(s) :

Progressive motility
AKAP4
Horse
Semen analysis
Cryopreservation

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

A-kinase anchor protein 4 (AKAP4) is playing a central role in flagellar structure, chemotaxis, capacitation and sperm motility. In mammals, AKAP4 is expressed during spermatogenesis. AKAP4 is synthesized as a precursor, ...
Show more >A-kinase anchor protein 4 (AKAP4) is playing a central role in flagellar structure, chemotaxis, capacitation and sperm motility. In mammals, AKAP4 is expressed during spermatogenesis. AKAP4 is synthesized as a precursor, proAKAP4, which is cleaved into mature AKAP4 during fibrous sheath assembly. The proAKAP4 is a good indicator of sperm quality in humans and boars. The aims of this work were to study the expression, the localization and the concentration of proAKAP4 and AKAP4 in equine semen, and to evaluate the possible correlation between the total and progressive motility and the concentration of proAKAP4 measured by ELISA in post-thawed semen. Frozen sperm from 13 different stallions were used. Semen samples (n = 17) were prepared using the INRA Freeze medium to reach a concentration of 150 million spermatozoa/mL, packaged and frozen in 0.5 mL straws. The precursor proAKAP4 and the mature protein AKAP4 both localize to the fibrous sheath of the principle piece of equine sperm flagellum. The concentrations of proAKAP4 were determined in the post-thawed semen using ELISA method (Horse 4MID®Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

• Lille Neurosciences & Cognition (LilNCog) - U 1172

Research team(s) :

Alzheimer et Tauopathies

Submission date :

2021-06-23T13:42:48Z