Titre :

Heterologous biosynthesis of five new class II bacteriocins from Lactobacillus paracasei CNCM I-5369 with antagonistic activity against pathogenic Escherichia coli strains

Auteur(s) :

Belguesmia, Yanath [Auteur correspondant]
BioEcoAgro - Equipe 8 - Food and Digestive Microbial Ecosystems: Interactions - Dynamics - Application(s)
Bendjeddou, Kamel [Auteur]
Université Abderrahmane Mira [Université de Béjaïa] = University of Béjaïa = جامعة بجاية [UB]
Kempf, Isabelle [Auteur]
Mycoplasmologie, Bactériologie et Antibiorésistance [MBA]
Laboratoire de Ploufragan-Plouzané-Niort [ANSES]
Boukherroub, Rabah [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Drider, Djamel [Auteur]
BioEcoAgro - Equipe 8 - Food and Digestive Microbial Ecosystems: Interactions - Dynamics - Application(s)
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]

Titre de la revue :

Frontiers in Microbiology

Pagination :

1198

Éditeur :

Frontiers Media

Date de publication :

2020-06

ISSN :

1664-302X

Mot(s)-clé(s) en anglais :

Lactobacillus paracasei
Escherichia coli
Antimicrobial agents
multibacteriocinogenic strain
bacteriocins

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Alimentation et Nutrition

Résumé en anglais : [en]

Lactobacillus paracasei CNCM I-5369 isolated from a traditional Algerian dairy product produces extracellular inhibitory substances, namely, bacteriocins, which are active against a panel of pathogenic Escherichia coli ...
Lire la suite >Lactobacillus paracasei CNCM I-5369 isolated from a traditional Algerian dairy product produces extracellular inhibitory substances, namely, bacteriocins, which are active against a panel of pathogenic Escherichia coli strains. This activity was observed only at a narrow pH 4.5-5, and resulted to be heat stable and sensitive to the action of proteolytic enzymes, which indicate a proteinaceous nature. This new strain has a genome of 2,752,975 bp, with a 46.6% G + C ratio and contains at least 2664 coding sequences. The Bagel software analysis identified five open reading frames (ORFs) that are translated to new class II bacteriocin. Each ORF was cloned in frame with a His-tag tail and expressed in E. coli BL21 (DE3) (pLysS) strain. Of note, each fusion protein carrying any of these ORFs at the C- or N-terminal position resulted to be active against E. coli 184 strain used as target organism. This manuscript reports the first multi-bacteriocinogenic strain producing five new class II bacteriocins with activity against Gram-negative bacilli (GNB), namely, E. coli. Heterologous expression and activity of each new class II bacteriocin were demonstrated.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

Alternatives stratégiques pour réduire l'usage de la colistine dans les pratiques d'élevage de porc

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL