Cell detachment and label-free cell sorting using modulated surface acoustic waves (SAW) in droplet-based microfluidics

Bussonnière, Adrien; Miron, Yannick; Baudoin, Michael; Bou Matar, Olivier; Grandbois, Michel; Charette, Paul; Renaudin, Alan

Document type :

Article dans une revue scientifique

DOI :

10.1039/C4LC00625A

Title :

Cell detachment and label-free cell sorting using modulated surface acoustic waves (SAW) in droplet-based microfluidics

Author(s) :

Bussonnière, Adrien [Auteur]
Miron, Yannick [Auteur]
Baudoin, Michael [Auteur]

Bou Matar, Olivier [Auteur]
Grandbois, Michel [Auteur]
Charette, Paul [Auteur]
Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] [3IT]
Laboratoire Nanotechnologies et Nanosystèmes [Sherbrooke] [LN2]
Renaudin, Alan [Auteur]
Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] [3IT]
Laboratoire Nanotechnologies et Nanosystèmes [Sherbrooke] [LN2]

Journal title :

Lab on a Chip

Pages :

3556-3563

Publisher :

Royal Society of Chemistry

Publication date :

2014

ISSN :

1473-0197

HAL domain(s) :

Physique [physics]/Mécanique [physics]/Mécanique des fluides [physics.class-ph]
Physique [physics]/Mécanique [physics]/Acoustique [physics.class-ph]
Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph]

English abstract : [en]

We present a droplet-based surface acoustic wave (SAW) system designed to viably detach biological cells from a surface and sort cell types based on differences in adhesion strength (adhesion contrast) without the need to ...
Show more >We present a droplet-based surface acoustic wave (SAW) system designed to viably detach biological cells from a surface and sort cell types based on differences in adhesion strength (adhesion contrast) without the need to label cells with molecular markers. The system uses modulated SAW to generate pulsatile flows in the droplets and efficiently detach the cells, thereby minimizing the SAW excitation power and exposure time. As a proof of principle, the system shows efficient sorting of HEK 293 from A7r5 cells based on adhesion contrast. Results are obtained in minutes with sorting purity and efficiency reaching 97% and 95%, respectively.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Non spécifiée

Popular science :

Non

ANR Project :

Développement de stratégies de fabrication par hybridation des procédés WXAM et usinage 5 axes de formes complexes

Collections :