Titre :

Dual-Color Bioluminescence Imaging for Simultaneous Monitoring of the Intestinal Persistence of Lactobacillus plantarum and Lactococcus lactis in Living Mice

Auteur(s) :

Daniel, Catherine [Auteur correspondant]
Lactic Acid Bacteria & Mucosal Immunity - CIIL
Poiret, Sabine [Auteur]
Lactic Acid Bacteria & Mucosal Immunity - CIIL
Dennin, Véronique [Auteur]
Lactic Acid Bacteria & Mucosal Immunity - CIIL
Boutillier, Denise [Auteur]
Lactic Acid Bacteria & Mucosal Immunity - CIIL
Lacorre, Delphine Armelle [Auteur]
Microbiologie cellulaire et physique de l’infection = Cellular Microbiology and Physics of Infection Group [Lille] [CMPI]
Plateforme BioImaging Center Lille - PLBS [BICeL]
Foligné, Benoit [Auteur]
Lactic Acid Bacteria & Mucosal Immunity - CIIL
Pot, Bruno [Auteur]
Lactic Acid Bacteria & Mucosal Immunity - CIIL

Titre de la revue :

Applied and Environmental Microbiology

Pagination :

5344-5349

Éditeur :

American Society for Microbiology

Date de publication :

2015-07-21

ISSN :

0099-2240

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Lactic acid bacteria are found in the gastrointestinal tract of mammals and have received tremendous attention due to their health-promoting properties. We report the development of two dual-color luciferase-producing ...
Lire la suite >Lactic acid bacteria are found in the gastrointestinal tract of mammals and have received tremendous attention due to their health-promoting properties. We report the development of two dual-color luciferase-producing Lactobacillus ( Lb .) plantarum and Lactococcus ( Lc .) lactis strains for noninvasive simultaneous tracking in the mouse gastrointestinal tract. We previously described the functional expression of the red luciferase mutant (CBRluc) from Pyrophorus plagiophthalamus in Lb. plantarum NCIMB8826 and Lc. lactis MG1363 (C. Daniel, S. Poiret, V. Dennin, D. Boutillier, and B. Pot, Appl Environ Microbiol 79: 1086–1094, 2013, http://dx.doi.org/10.1128/AEM.03221-12 ). In this study, we determined that CBRluc is a better-performing luciferase for in vivo localization of both lactic acid bacteria after oral administration than the green click beetle luciferase mutant construct developed in this study. We further established the possibility to simultaneously detect red- and green-emitting lactic acid bacteria by dual-wavelength bioluminescence imaging in combination with spectral unmixing. The difference in spectra of light emission by the red and green click beetle luciferase mutants and dual bioluminescence detection allowed in vitro and in vivo quantification of the red and green emitted signals; thus, it allowed us to monitor the dynamics and fate of the two bacterial populations simultaneously. Persistence and viability of both strains simultaneously administered to mice in different ratios was studied in vivo in anesthetized mice and ex vivo in mouse feces. The application of dual-luciferase-labeled bacteria has considerable potential to simultaneously study the interactions and potential competitions of different targeted bacteria and their hosts.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL