Probing nanoparticle-membrane interactions ...
Document type :
Compte-rendu et recension critique d'ouvrage
DOI :
PMID :
Title :
Probing nanoparticle-membrane interactions by combining amphiphilic diblock copolymer assembly and plasmonics
Author(s) :
Koch, Amelie H. R. [Auteur]
Max Planck Institute for Polymer Research
Morsbach, Svenja [Auteur]
Max Planck Institute for Polymer Research
Bereau, Tristan [Auteur]
Max Planck Institute for Polymer Research
Leveque, Gaetan [Auteur]
Physique - IEMN [PHYSIQUE - IEMN]
Butt, Hans-Juergen [Auteur]
Max Planck Institute for Polymer Research
Deserno, Markus [Auteur]
Machine Learning Department [Carnegie Mellon Univ.]
Landfester, Katharina [Auteur]
Max Planck Institute for Polymer Research
Fytas, George [Auteur correspondant]
Max Planck Institute for Polymer Research
Max Planck Institute for Polymer Research
Morsbach, Svenja [Auteur]
Max Planck Institute for Polymer Research
Bereau, Tristan [Auteur]
Max Planck Institute for Polymer Research
Leveque, Gaetan [Auteur]
Physique - IEMN [PHYSIQUE - IEMN]
Butt, Hans-Juergen [Auteur]
Max Planck Institute for Polymer Research
Deserno, Markus [Auteur]
Machine Learning Department [Carnegie Mellon Univ.]
Landfester, Katharina [Auteur]
Max Planck Institute for Polymer Research
Fytas, George [Auteur correspondant]
Max Planck Institute for Polymer Research
Journal title :
Journal of Physical Chemistry B
Pages :
742-750
Publisher :
American Chemical Society
Publication date :
2020
ISSN :
1520-6106
HAL domain(s) :
Chimie/Polymères
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Understanding the interactions between nanoparticles (NPs) and boundaries of cells is crucial both for their toxicity and therapeutic applications. Besides specific receptor-mediated endocytosis of surface-functionalized ...
Show more >Understanding the interactions between nanoparticles (NPs) and boundaries of cells is crucial both for their toxicity and therapeutic applications. Besides specific receptor-mediated endocytosis of surface-functionalized NPs, passive internalization is prompted by relatively unspecific parameters, such as particle size and charge. Based on theoretical treatments, adhesion to and bending of the cell membrane can induce NP wrapping. Experimentally, powerful tools are needed to selectively probe possible membrane-NP motifs at very dilute conditions and avoid dye labeling. In this work, we employ surface resonance-enhanced dynamic light scattering, surface plasmon resonance, electron microscopy, and simulations for sensing interactions between plasmonic AuNPs and polymersomes. We distinguish three different interaction scenarios at nanomolar concentrations by tuning the surface charge of AuNPs and rationalize these events by balancing vesicle bending and electrostatic/van der Waals AuNP and vesicle adhesion. The clarification of the physical conditions under which nanoparticles passively translocate across membranes can aid in the rational design of drugs that cannot exploit specific modes of cellular uptake and also elucidates physical properties that render nanoparticles in the environment particularly toxic.Show less >
Show more >Understanding the interactions between nanoparticles (NPs) and boundaries of cells is crucial both for their toxicity and therapeutic applications. Besides specific receptor-mediated endocytosis of surface-functionalized NPs, passive internalization is prompted by relatively unspecific parameters, such as particle size and charge. Based on theoretical treatments, adhesion to and bending of the cell membrane can induce NP wrapping. Experimentally, powerful tools are needed to selectively probe possible membrane-NP motifs at very dilute conditions and avoid dye labeling. In this work, we employ surface resonance-enhanced dynamic light scattering, surface plasmon resonance, electron microscopy, and simulations for sensing interactions between plasmonic AuNPs and polymersomes. We distinguish three different interaction scenarios at nanomolar concentrations by tuning the surface charge of AuNPs and rationalize these events by balancing vesicle bending and electrostatic/van der Waals AuNP and vesicle adhesion. The clarification of the physical conditions under which nanoparticles passively translocate across membranes can aid in the rational design of drugs that cannot exploit specific modes of cellular uptake and also elucidates physical properties that render nanoparticles in the environment particularly toxic.Show less >
Language :
Anglais
Popular science :
Non
Source :
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