Titre :

In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice

Auteur(s) :

Cuffaro, Bernardo [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Assohoun, Aka [Auteur]
Université Nangui Abrogoua [UNA]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Boutillier, Denise [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Súkeníková, Lenka [Auteur]
Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic] [UK]
Desramaut, Jérémy [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Boudebbouze, Samira [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Salomé-Desnoulez, Sophie [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Hrdý, Jiří [Auteur]
Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic] [UK]
Waligora-Dupriet, Anne-Judith [Auteur]
Physiopathologie et pharmacotoxicologie placentaire humaine : Microbiote pré & post natal [3PHM - UMR-S 1139]
Maguin, Emmanuelle [Auteur correspondant]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Grangette, Corinne [Auteur correspondant]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Titre de la revue :

Cells

Pagination :

2104

Éditeur :

MDPI

Date de publication :

2020

ISSN :

2073-4409

Mot(s)-clé(s) en anglais :

Colitis
Microbiota composition
probiotics
Holobionts
biotherapeutic products
immune response
live
functional screening

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of ...
Lire la suite >Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five Parabacteroides distasonis strains isolated from human adult and neonates gut microbiota. We evaluated in vitro their immunomodulation capacities and their ability to reinforce the gut barrier and characterized in vivo their protective effects in an acute murine model of colitis. The in vitro beneficial activities were highly strain dependent: two strains exhibited a potent anti-inflammatory potential and restored the gut barrier while a third strain reinstated the epithelial barrier. While their survival to in vitro gastric conditions was variable, the levels of P. distasonis DNA were higher in the stools of bacteria-treated animals. The strains that were positively scored in vitro displayed a strong ability to rescue mice from colitis. We further showed that two strains primed dendritic cells to induce regulatory T lymphocytes from naïve CD4+ T cells. This study provides better insights on the functionality of commensal bacteria and crucial clues to design live biotherapeutics able to target inflammatory chronic diseases such as IBD.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL