Titre :

Molecular Characterization of Carbapenem-Nonsusceptible Enterobacterial Isolates Collected during a Prospective Interregional Survey in France and Susceptibility to the Novel Ceftazidime-Avibactam and Aztreonam-Avibactam Combinations

Auteur(s) :

Dupont, Herve [Auteur]
Mécanismes physiopathologiques et conséquences des calcifications cardiovasculaires - UR UPJV 7517 [MP3CV]
CHU Amiens-Picardie
Gaillot, Olivier [Auteur]
Goetgheluck, Anne-Sophie [Auteur]
Plassart, Claire [Auteur]
Emond, Jean-Philippe [Auteur]
Centre Hospitalier Compiègne-Noyon
Lecuru, Marion [Auteur]
CHU Amiens-Picardie
Gaillard, Nicolas [Auteur]
CHU Amiens-Picardie
Derdouri, Sarah [Auteur]
CHU Amiens-Picardie
Lemaire, Baptiste [Auteur]
CHU Amiens-Picardie
Courtilles, Marion Girard [Auteur]
CHU Amiens-Picardie
Cattoir, Vincent [Auteur]
Unité de Recherche Risques Microbiens [U2RM]
Service de Microbiologie [CHU Caen]
Mammeri, Hedi [Auteur]
Infection, Anti-microbiens, Modélisation, Evolution [IAME (UMR_S_1137 / U1137)]
CHU Amiens-Picardie

Titre de la revue :

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Pagination :

215-221

Date de publication :

2016

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

An interregional surveillance program was conducted in the northwestern part of France to determine the prevalence of carbapenem-nonsusceptible Enterobacteriaceae (CNSE) isolates and their susceptibility to ceftazidime-avibactam ...
Lire la suite >An interregional surveillance program was conducted in the northwestern part of France to determine the prevalence of carbapenem-nonsusceptible Enterobacteriaceae (CNSE) isolates and their susceptibility to ceftazidime-avibactam and aztreonam-avibactam combinations. Nonduplicate CNSE clinical isolates were prospectively collected from six hospitals between June 2012 and November 2013. MICs of ceftazidime and aztreonam, alone or combined with a fixed concentration of avibactam (4 mu g/ml), and those of carbapenems (comparator agents) were determined. MICs of ertapenem in combination with phenylalanine arginine-naphthylamide dihydrochloride (PA beta N) were also determined to assess active efflux. Genes encoding carbapenemases, plasmid-mediated AmpC enzymes, extended-spectrum beta-lactamases (ESBLs), and major outer membrane proteins (OMPs) were amplified and sequenced. OMPs were also extracted for SDS-PAGE analysis. Among the 139 CNSE isolates, mainly Enterobacter spp. and Klebsiella pneumoniae, 123 (88.4%) were ertapenem nonsusceptible, 12 (8.6%) exhibited reduced susceptibility to all carbapenems, and 4 Proteeae isolates (2.9%) were resistant to imipenem. Carbapenemase production was detected in only two isolates (producing OXA-48 and IMI-3). In contrast, OMP deficiency, in association with AmpCs and/or ESBLs (mainly CTX-M-9, SHV-12, and CTX-M-15), was largely identified among CNSE isolates. The ceftazidime-avibactam and aztreonam-avibactam combinations exhibited potent activity against CNSE isolates (MIC50/MIC90, 1/1 mu g/ml and 0.5/0.5 mu g/ml, respectively) compared to that of ceftazidime and aztreonam alone (MIC50/MIC90, 512/512 mu g/ml and 128/512 mu g/ml, respectively). This study reveals the in vitro activity of ceftazidime-avibactam and aztreonam-avibactam combinations against a large collection of porin-deficient enterobacterial isolates that are representative of the CNSE recovered in the northern part of France.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL

Date de dépôt :

2022-02-18T02:30:42Z