Evaluation of amazonian fungi biomass as ...
Type de document :
Article dans une revue scientifique: Article original
URL permanente :
Titre :
Evaluation of amazonian fungi biomass as a source of lipases for biocatalysis
Auteur(s) :
Romano, Israel P. [Auteur]
Dos Santos, Vanderlei S. [Auteur]
De Lima Paes Louzada, Ana Carolina [Auteur]
Pereira, Junior Raimundo C. [Auteur]
Do Carmo, Edson J. [Auteur]
Da Mota, Adolfo Jose [Auteur]
Barroso, Hileia Dos S. [Auteur]
Itabaiana, Ivaldo [Auteur]
Pereira, Jose Odair [Auteur]
Astolfi, Filho Spartaco [Auteur]
Zanotto, Sandra Patricia [Auteur]
Dos Santos, Vanderlei S. [Auteur]
De Lima Paes Louzada, Ana Carolina [Auteur]
Pereira, Junior Raimundo C. [Auteur]
Do Carmo, Edson J. [Auteur]
Da Mota, Adolfo Jose [Auteur]
Barroso, Hileia Dos S. [Auteur]
Itabaiana, Ivaldo [Auteur]
Pereira, Jose Odair [Auteur]
Astolfi, Filho Spartaco [Auteur]
Zanotto, Sandra Patricia [Auteur]
Titre de la revue :
Quimica nova
Nom court de la revue :
Quim. Nova
Numéro :
43
Pagination :
146-154
Date de publication :
2020-02-03
ISSN :
0100-4042
Mot(s)-clé(s) en anglais :
transesterification
amazonian fungi
enantioselectivity
biocatalysis
lipase
biomass
amazonian fungi
enantioselectivity
biocatalysis
lipase
biomass
Discipline(s) HAL :
Chimie/Catalyse
Résumé en anglais : [en]
We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic ...
Lire la suite >We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ees = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ees = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.Lire moins >
Lire la suite >We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ees = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ees = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
CNRS
Centrale Lille
ENSCL
Univ. Artois
Université de Lille
Centrale Lille
ENSCL
Univ. Artois
Université de Lille
Collections :
Date de dépôt :
2022-03-02T07:14:36Z