Titre :

Modulation of designer biomimetic matrices for optimized differentiated intestinal epithelial cultures

Auteur(s) :

Xi, Wang [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]
Saleh, Jad [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]
Yamada, Ayako [Auteur]
Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) [PASTEUR]
Tomba, Caterina [Auteur]
INL - Dispositifs pour la Santé et l’Environnement [INL - DSE]
Mercier, Barbara [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]
Janel, Sébastien [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Dang, Tien [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]
Soleilhac, Matis [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]
Djemat, Aurélie [Auteur]
Wu, Huiqiong [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]
Romagnolo, Béatrice [Auteur]
[Institut Cochin] Département Développement, Reproduction et Cancer [DRC]
Lafont, Frank [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Mège, René-Marc [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]
Chen, Yong [Auteur]
Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) [PASTEUR]
Delacour, Delphine [Auteur]
Institut Jacques Monod [IJM (UMR_7592)]

Titre de la revue :

Biomaterials

Pagination :

121380

Éditeur :

Elsevier

Date de publication :

2022-03

ISSN :

0142-9612

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

The field of intestinal biology is thirstily searching for different culture methods that complement the limitations of organoids, particularly the lack of a differentiated intestinal compartment. While being recognized ...
Lire la suite >The field of intestinal biology is thirstily searching for different culture methods that complement the limitations of organoids, particularly the lack of a differentiated intestinal compartment. While being recognized as an important milestone for basic and translational biological studies, many primary cultures of intestinal epithelium (IE) rely on empirical trials using hydrogels of various stiffness, whose mechanical impact on epithelial organization remains vague until now. Here, we report the development of hydrogel scaffolds with a range of elasticities and their influence on IE expansion, organization, and differentiation. On stiff substrates (>5 kPa), mouse IE cells adopt a flat cell shape and detach in the short-term. In contrast, on soft substrates (80-500 Pa), they sustain for a long-term, pack into high density, develop columnar shape with improved apical-basal polarity and differentiation marker expression, a phenotype reminiscent of features in vivo mouse IE. We then developed a soft gel molding process to produce 3D Matrigel scaffolds of close-to-nature stiffness, which support and maintain a culture of mouse IE into crypt-villus architecture. Thus, the present work is up-to-date informative for the design of biomaterials for ex vivo intestinal models, offering self-renewal in vitro culture that emulates the mouse IE.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

Université de Paris

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL