Titre :

Molecular characterization and mRNA expression of grp78 and hsp90A in the estuarine copepod Eurytemora affinis

Auteur(s) :

Xuereb, Benoit [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]
Forget-Leray, J. [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]
Souissi, Sami [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Glippa, O. [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Devreker, D. [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Lesueur, Teddy [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]
Marie, Sabine [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]
Danger, Jean-Michel [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]
Boulangé-Lecomte, Celine [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]

Titre de la revue :

Eurytemora affinis.

Pagination :

457-472

Date de publication :

2012-07

Mot(s)-clé(s) :

MYTILUS-GALLOPROVINCIALIS

Mot(s)-clé(s) en anglais :

Abiotic stress
Copepod
Estuary
Glucose-regulated protein 78
Heat shock protein 90
HEAT-SHOCK PROTEINS
REAL-TIME PCR
SEINE ESTUARY
RIMICARIS-EXOCULATA
GENE-EXPRESSION
STRESS-PROTEINS
BINDING-SITE
FRESH-WATER
HSP70

Discipline(s) HAL :

Planète et Univers [physics]/Sciences de la Terre/Océanographie

Résumé en anglais : [en]

The present study aimed to develop a method of quantification of heat shock protein transcript levels in the estuarine copepod Eurytemora affinis. For that, the full-length cDNA of the 78-kDa glucose-regulated protein ...
Lire la suite >The present study aimed to develop a method of quantification of heat shock protein transcript levels in the estuarine copepod Eurytemora affinis. For that, the full-length cDNA of the 78-kDa glucose-regulated protein (Ea-grp78) and the cytosolic 90-kDa heat shock protein (Ea-hsp90A) from this species have been cloned. These cDNA revealed, respectively, 2,370 and 2,299 bp with 1,971 and 2,124 bp open reading frames encoding 656 and 707 amino acids. Main features, sequence identities and phylogenetic analysis with other species were described. Then, the expression profiles were analysed using reverse transcription/real-time quantitative PCR method from copepods subjected to different thermic and osmotic stresses in laboratory, and from copepods directly sampled into the natural population of the Seine Estuary (France) along a salinity gradient. Thermic shock (7.5°C, 22.5°C and 30°C during 90 min) significantly induced increases of transcript quantities ranged between 1.7- and 19.7-fold the levels observed in control conditions (15°C). Hypo- and hyper-osmotic shocks (salinities of 1 and 30 during 90 min) caused a 2-fold induction of Ea-hsp90A transcript level in comparison to controls (salinity of 15) whereas no significant change was measured for Ea-grp78. On the other hand, similar expression profiles were observed for the two transcripts after 72 h of exposition to salinities of 1 and 25 with a significant 2-fold induction observed for the lower salinity. To finish, strong expression inductions of both Ea-grp78 and Ea-hsp90A genes were observed in field copepods sampled at low salinity during the campaigns of June 2009 and May 2010. These results tend to show that the low salinity and the increase of temperature seem to have a synergic effect on stress condition of copepods.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Laboratoire d'Océanologie et de Géosciences (LOG) - UMR 8187

Source :

Harvested from HAL