Title :

OTX2 stimulates adult retinal ganglion cell regeneration

Author(s) :

Ibad, Raoultorero [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Quenech’du, Nicole [Auteur]
Centre interdisciplinaire de recherche en biologie [CIRB]
Prochiantz, Alain [Auteur]
Institute of Neurosciences
Collège de France [CdF (institution)]
Moya, Kennethl [Auteur]
Centre interdisciplinaire de recherche en biologie [CIRB]

Journal title :

Neural Regeneration Research

Pages :

690

Publisher :

Publishing House of Neural Regeneration Research

Publication date :

2022

ISSN :

1673-5374

English keyword(s) :

GAP-43
OTX2
axon regeneration
dissociated retinal culture
homeoprotein
optic nerve crush
retinal explants
retinal ganglion cell

HAL domain(s) :

Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]/Neurobiologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Sciences du Vivant [q-bio]/Biologie du développement
Sciences du Vivant [q-bio]/Biologie cellulaire

English abstract : [en]

Retinal ganglion cell (RGC) axons provide the only link between the light sensitive and photon transducing neural retina and visual centers of the brain. RGC axon degeneration occurs in a number of blinding diseases and ...
Show more >Retinal ganglion cell (RGC) axons provide the only link between the light sensitive and photon transducing neural retina and visual centers of the brain. RGC axon degeneration occurs in a number of blinding diseases and the ability to stimulate axon regeneration from surviving ganglion cells could provide the anatomic substrate for restoration of vision. OTX2 is a homeoprotein transcription factor expressed in the retina and previous studies showed that, in response to stress, exogenous OTX2 increases the in vitro and in vivo survival of RGCs. Here we examined and quantified the effects of OTX2 on adult RGC axon regeneration in vitro and in vivo. The results show that exogenous OTX2 stimulates the regrowth of axons from RGCs in cultures of dissociated adult retinal cells and from explants of adult retinal tissue and that RGCs respond directly to OTX2 as regrowth is observed in cultures of purified adult rat RGCs. Importantly, after nerve crush in vivo, we observed a positive effect of OTX2 on the number of regenerating axons up to the optic chiasm within 14 days post crush and a very modest level of acuity absent in control mice. The effect of OTX2 on RGC survival and regeneration is of potential interest for degenerative diseases affecting this cell type. All animal procedures were approved by the local "Comié d'éιthique en expérimentation animale n°59" and authorization n° 00702.01 delivered March 28, 2014 by the French "Ministére de l'enseignement supérieur et de la recherche".Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL