Title :

Characteristic ERK1/2 signaling dynamics distinguishes necroptosis from apoptosis

Author(s) :

Sipieter, François [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
Université de Lille
Institut Jacques Monod [IJM (UMR_7592)]
Cappe, Benjamin [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
Leray, Aymeric [Auteur]
Laboratoire Interdisciplinaire Carnot de Bourgogne [ICB]
de Schutter, Elke [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
University of Antwerp [UA]
Bridelance, Jolien [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
Hulpiau, Paco [Auteur]
Camp, Guy [Auteur]
University of Antwerp [UA]
Antwerp University Hospital [Edegem] [UZA]
Declercq, Wim [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
Héliot, Laurent [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Vincent, Pierre [Auteur]
Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing [B2A-IBPS]
Vandenabeele, Peter [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]
Riquet, Franck [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Université de Lille
VIB-UGent Center for Inflammation Research [Gand, Belgique] [IRC]

Journal title :

ISCIENCE

Pages :

103074

Publisher :

Elsevier

Publication date :

2021-09

English keyword(s) :

Biological sciences
Biomolecular engineering
Cell biology

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

ERK1/2 involvement in cell death remains unclear, although many studies have demonstrated the importance of ERK1/2 dynamics in determining cellular re- sponses. To untangle how ERK1/2 contributes to two cell death programs, ...
Show more >ERK1/2 involvement in cell death remains unclear, although many studies have demonstrated the importance of ERK1/2 dynamics in determining cellular re- sponses. To untangle how ERK1/2 contributes to two cell death programs, we investigated ERK1/2 signaling dynamics during hFasL-induced apoptosis and TNF-induced necroptosis in L929 cells. We observed that ERK1/2 inhibition sensi- tizes cells to apoptosis while delaying necroptosis. By monitoring ERK1/2 activity by live-cell imaging using an improved ERK1/2 biosensor (EKAR4.0), we reported differential ERK1/2 signaling dynamics between cell survival, apoptosis, and nec- roptosis. We also decrypted a temporally shifted amplitude- and frequency-modu- lated (AM/FM) ERK1/2 activity profile in necroptosis versus apoptosis. ERK1/2 inhibition, which disrupted ERK1/2 signaling dynamics, prevented TNF and IL-6 gene expression increase during TNF-induced necroptosis. Using an inducible cell line for activated MLKL, the final executioner of necroptosis, we showed ERK1/2 and its distinctive necroptotic ERK1/2 activity dynamics to be positioned downstream of MLKL.Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Regulation et rôles des kinases ERK1&2 (Extracelular Regulated Kinase 1&2) and Plk1 (Polo-like kinase 1) dans la progression G2/mitose

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL