Title :

SARS-CoV-2 detection using a nanobody-functionalized voltammetric device

Author(s) :

Pagneux, Quentin [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Roussel, Alain [Auteur correspondant]
Architecture et fonction des macromolécules biologiques [AFMB]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Saada, Hiba [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Cambillau, Christian [Auteur]
Architecture et fonction des macromolécules biologiques [AFMB]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Amigues, Béatrice [Auteur]
Architecture et fonction des macromolécules biologiques [AFMB]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Delauzun, Vincent [Auteur]
Architecture et fonction des macromolécules biologiques [AFMB]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Engelmann, Ilka [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pathogenesis and Control of Chronic and Emerging Infections [PCCEI]
Alidjinou, Enagnon Kazali []
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Ogiez, Judith [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Rolland, Anne Sophie [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Poissy, Julien [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Duhamel, Alain [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
NanoBioInterfaces - IEMN [NBI - IEMN]
Devos, David [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
NanoBioInterfaces - IEMN [NBI - IEMN]

Journal title :

Communications Medicine

Pages :

56

Publisher :

Nature

Publication date :

2022-12

English keyword(s) :

Diagnostic markers
Nanobiotechnology

HAL domain(s) :

Sciences de l'ingénieur [physics]
Sciences du Vivant [q-bio]

English abstract : [en]

Background: An ongoing need during the COVID-19 pandemic has been the requirement for accurate and efficient point-of-care testing platforms to distinguish infected from non-infected people, and to differentiate SARS-CoV-2 ...
Show more >Background: An ongoing need during the COVID-19 pandemic has been the requirement for accurate and efficient point-of-care testing platforms to distinguish infected from non-infected people, and to differentiate SARS-CoV-2 infections from other viruses. Electrochemical platforms can detect the virus via its envelope spike protein by recording changes in voltammetric signals between samples. However, this remains challenging due to the limited sensitivity of these sensing platforms.Methods: Here, we report on a nanobody-functionalized electrochemical platform for the rapid detection of whole SARS-CoV-2 viral particles in complex media such as saliva and nasopharyngeal swab samples. The sensor relies on the functionalization of gold electrode surface with highly-oriented Llama nanobodies specific to the spike protein receptor binding domain (RBD). The device provides results in 10 min of exposure to 200 µL of unprocessed samples with high specificity to SARS-CoV-2 viral particles in human saliva and nasopharyngeal swab samples.Results: The developed sensor could discriminate between different human coronavirus strains and other respiratory viruses, with 90% positive and 90% negative percentage agreement on 80 clinical samples, as compared to RT-qPCR.Conclusions: We believe this diagnostic concept, also validated for RBD mutants and successfully tested on Delta variant samples, to be a powerful tool to detect patients' infection status, easily extendable to other viruses and capable of overcoming sensing-related mutation effects.Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Infrastructure Française pour la Biologie Structurale Intégrée
Dispositive portable de diagnostic pour différencier le virus la grippe de celui de Covid-19

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL