Tankyrase regulates epithelial lumen ...
Type de document :
Article dans une revue scientifique: Article original
DOI :
PMID :
URL permanente :
Titre :
Tankyrase regulates epithelial lumen formation via suppression of rab11 gefs
Auteur(s) :
Chandrakumar, Arun A. [Auteur]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Coyaud, Etienne-Marie [Auteur]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Marshall, Christopher B. [Auteur]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Ikura, Mitsuhiko [Auteur]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Raught, Brian [Auteur]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Rottapel, Robert [Auteur]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University of Toronto
St. Michael's Hospital
University Health Network [Toronto, ON, Canada]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Coyaud, Etienne-Marie [Auteur]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Marshall, Christopher B. [Auteur]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Ikura, Mitsuhiko [Auteur]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Raught, Brian [Auteur]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University Health Network [Toronto, ON, Canada]
Rottapel, Robert [Auteur]
Department of Medical Biophysics [MBP]
Princess Margaret Hospital
University of Toronto
St. Michael's Hospital
University Health Network [Toronto, ON, Canada]
Titre de la revue :
Journal of Cell Biology
Nom court de la revue :
J. Cell Biol.
Numéro :
220
Pagination :
e202008037
Éditeur :
Rockefeller University Press
Date de publication :
2021-07-05
ISSN :
0021-9525
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Rab11 GTPase proteins are required for cytokinesis, ciliogenesis, and lumenogenesis. Rab11a is critical for apical delivery of podocalyxin (PODXL) during lumen formation in epithelial cells. SH3BP5 and SH3BP5L are guanine ...
Lire la suite >Rab11 GTPase proteins are required for cytokinesis, ciliogenesis, and lumenogenesis. Rab11a is critical for apical delivery of podocalyxin (PODXL) during lumen formation in epithelial cells. SH3BP5 and SH3BP5L are guanine nucleotide exchange factors (GEFs) for Rab11. We show that SH3BP5 and SH3BP5L are required for activation of Rab11a and cyst lumen formation. Using proximity-dependent biotin identification (BioID) interaction proteomics, we have identified SH3BP5 and its paralogue SH3BP5L as new substrates of the poly-ADP-ribose polymerase Tankyrase and the E3 ligase RNF146. We provide data demonstrating that epithelial polarity via cyst lumen formation is governed by Tankyrase, which inhibits Rab11a activation through the suppression of SH3BP5 and SH3BP5L. RNF146 reduces Tankyrase protein abundance and restores Rab11a activation and lumen formation. Thus, Rab11a activation is controlled by a signaling pathway composed of the sequential inhibition of SH3BP5 paralogues by Tankyrase, which is itself suppressed by RNF146.Lire moins >
Lire la suite >Rab11 GTPase proteins are required for cytokinesis, ciliogenesis, and lumenogenesis. Rab11a is critical for apical delivery of podocalyxin (PODXL) during lumen formation in epithelial cells. SH3BP5 and SH3BP5L are guanine nucleotide exchange factors (GEFs) for Rab11. We show that SH3BP5 and SH3BP5L are required for activation of Rab11a and cyst lumen formation. Using proximity-dependent biotin identification (BioID) interaction proteomics, we have identified SH3BP5 and its paralogue SH3BP5L as new substrates of the poly-ADP-ribose polymerase Tankyrase and the E3 ligase RNF146. We provide data demonstrating that epithelial polarity via cyst lumen formation is governed by Tankyrase, which inhibits Rab11a activation through the suppression of SH3BP5 and SH3BP5L. RNF146 reduces Tankyrase protein abundance and restores Rab11a activation and lumen formation. Thus, Rab11a activation is controlled by a signaling pathway composed of the sequential inhibition of SH3BP5 paralogues by Tankyrase, which is itself suppressed by RNF146.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
INSERM
Université de Lille
Université de Lille
Collections :
Date de dépôt :
2022-06-15T13:58:18Z
2023-03-08T13:22:09Z
2023-03-08T13:22:09Z
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