Title :

Laser-assisted rapid evaporative ionisation mass spectrometry (la-reims) as a metabolomics platform in cervical cancer screening

Author(s) :

Paraskevaidi, Maria [Auteur]
Imperial College London
Cameron, Simon J. S. [Auteur]
Imperial College London
Whelan, Eilbhe [Auteur]
Imperial College London
Bowden, Sarah [Auteur]
Imperial College London
Tzafetas, Menelaos [Auteur]
Imperial College London
Mitra, Anita [Auteur]
Imperial College London
Semertzidou, Anita [Auteur]
Imperial College London
Athanasiou, Antonis [Auteur]
Imperial College London
Bennett, Phillip R. [Auteur]
Imperial College London
Macintyre, David A. [Auteur]
Imperial College London
Takats, Zoltan [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Kyrgiou, Maria [Auteur]
Imperial College London

Journal title :

EBioMedicine

Abbreviated title :

EBioMedicine

Volume number :

60

Pages :

103017

Publisher :

Elsevier

Publication date :

2020-10-01

ISSN :

2352-3964

Keyword(s) :

Laser-assisted reims (LA-REIMS)
Cervical cancer screening
CIN
Cervical cancer
Liquid-based cytology
HPV tests
Metabolomics

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

BACKGROUND: The introduction of high-risk human papillomavirus (hrHPV) testing as part of primary cervical screening is anticipated to improve sensitivity, but also the number of women who will screen positive. Reflex ...
Show more >BACKGROUND: The introduction of high-risk human papillomavirus (hrHPV) testing as part of primary cervical screening is anticipated to improve sensitivity, but also the number of women who will screen positive. Reflex cytology is the preferred triage test in most settings but has limitations including moderate diagnostic accuracy, lack of automation, inter-observer variability and the need for clinician-collected sample. Novel, objective and cost-effective approaches are needed. METHODS: In this study, we assessed the potential use of an automated metabolomic robotic platform, employing the principle of laser-assisted Rapid Evaporative Ionisation Mass Spectrometry (LA-REIMS) in cervical cancer screening. FINDINGS: In a population of 130 women, LA-REIMS achieved 94% sensitivity and 83% specificity (AUC: 91.6%) in distinguishing women testing positive (n = 65) or negative (n = 65) for hrHPV. We performed further analysis according to disease severity with LA-REIMS achieving sensitivity and specificity of 91% and 73% respectively (AUC: 86.7%) in discriminating normal from high-grade pre-invasive disease. INTERPRETATION: This automated high-throughput technology holds promise as a low-cost and rapid test for cervical cancer screening and triage. The use of platforms like LA-REIMS has the potential to further improve the accuracy and efficiency of the current national screening programme. FUNDING: Work was funded by the MRC Imperial Confidence in Concept Scheme, Imperial College Healthcare Charity, British Society for Colposcopy and Cervical Pathology, National Research Development and Innovation Office of Hungary, Waters corporation and NIHR BRC.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T13:59:49Z
2023-04-05T08:05:11Z