Title :

Protein kinase c activation drives a differentiation program in an oligodendroglial precursor model through the modulation of specific biological networks

Author(s) :

Damato, Marina [Auteur]
Università del Salento = University of Salento [Lecce]
Cardon, Tristan [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Wisztorski (Admin), Maxence [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Fournier, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Pieragostino, Damiana [Auteur]
Università degli studi "G. d'Annunzio" Chieti-Pescara [Chieti-Pescara] [Ud'A]
Cicalini, Ilaria [Auteur]
Università degli studi "G. d'Annunzio" Chieti-Pescara [Chieti-Pescara] [Ud'A]
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Vergara, Daniele [Auteur]
Università del Salento = University of Salento [Lecce]
Maffia, Michele [Auteur]
Università del Salento = University of Salento [Lecce]

Journal title :

International Journal of Molecular Sciences

Abbreviated title :

Int J Mol Sci

Volume number :

22

Pages :

5245

Publication date :

2021-05-15

ISSN :

1422-0067

Keyword(s) :

PKC
differentiation
ROCK
cytoskeleton
mass spectrometry
signaling
oligodendrocytes

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Protein kinase C (PKC) activation induces cellular reprogramming and differentiation in various cell models. Although many effectors of PKC physiological actions have been elucidated, the molecular mechanisms regulating ...
Show more >Protein kinase C (PKC) activation induces cellular reprogramming and differentiation in various cell models. Although many effectors of PKC physiological actions have been elucidated, the molecular mechanisms regulating oligodendrocyte differentiation after PKC activation are still unclear. Here, we applied a liquid chromatography-mass spectrometry (LC-MS/MS) approach to provide a comprehensive analysis of the proteome expression changes in the MO3.13 oligodendroglial cell line after PKC activation. Our findings suggest that multiple networks that communicate and coordinate with each other may finally determine the fate of MO3.13 cells, thus identifying a modular and functional biological structure. In this work, we provide a detailed description of these networks and their participating components and interactions. Such assembly allows perturbing each module, thus describing its physiological significance in the differentiation program. We applied this approach by targeting the Rho-associated protein kinase (ROCK) in PKC-activated cells. Overall, our findings provide a resource for elucidating the PKC-mediated network modules that contribute to a more robust knowledge of the molecular dynamics leading to this cell fate transition.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Submission date :

2022-06-15T14:00:13Z
2023-02-01T10:43:15Z