Titre :

A High-Content Microscopy Screening Identifies New Genes Involved in Cell Width Control in Bacillus subtilis

Auteur(s) :

Juillot, Dimitri [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Cornilleau, Charlène [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Deboosere, Nathalie [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Billaudeau, Cyrille [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Evouna-Mengue, Parfait [Auteur]
MetaGenoPolis [MGP (US 1367)]
Lejard, Véronique [Auteur]
MetaGenoPolis [MGP (US 1367)]
Brodin, Priscille [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Carballido-López, Rut [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Chastanet, Arnaud [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]

Titre de la revue :

mSystems

Pagination :

pp. e01017-21

Date de publication :

2021

ISSN :

2379-5077

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie

Résumé en anglais : [en]

How cells control their shape and size is a fundamental question of biology. In most bacteria, cell shape is imposed by the peptidoglycan (PG) polymeric meshwork that surrounds the cell. Thus, bacterial cell morphogenesis ...
Lire la suite >How cells control their shape and size is a fundamental question of biology. In most bacteria, cell shape is imposed by the peptidoglycan (PG) polymeric meshwork that surrounds the cell. Thus, bacterial cell morphogenesis results from the coordinated action of the proteins assembling and degrading the PG shell. Remarkably, during steady-state growth, most bacteria maintain a defined shape along generations, suggesting that error-proof mechanisms tightly control the process. In the rod-shaped model for the Gram-positive bacterium Bacillus subtilis, the average cell length varies as a function of the growth rate, but the cell diameter remains constant throughout the cell cycle and across growth conditions. Here, in an attempt to shed light on the cellular circuits controlling bacterial cell width, we developed a screen to identify genetic determinants of cell width in B. subtilis. Using high-content screening (HCS) fluorescence microscopy and semiautomated measurement of single-cell dimensions, we screened a library of ∼4,000 single knockout mutants. We identified 13 mutations significantly altering cell diameter, in genes that belong to several functional groups. In particular, our results indicate that metabolism plays a major role in cell width control in B. subtilis.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

Plateau de microscopie de criblage à haut débit et d'analyse à très haute résolution

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL