Titre :

A novel mosquito species identification method based on PCR and capillary electrophoresis

Auteur(s) :

Chabanol, Estelle [Auteur]
Université de Guyane [UG]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Institut Pasteur de la Guyane
Romoli, Ottavia [Auteur]
Institut Pasteur de la Guyane
Talaga, Stanislas [Auteur]
Institut Pasteur de la Guyane
Epelboin, Yanouk [Auteur]
Institut Pasteur de la Guyane
Heu, Katy [Auteur]
Institut Pasteur de la Guyane
Prévot, Ghislaine [Auteur]
Université de Guyane [UG]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Gendrin, Mathilde [Auteur correspondant]
Institut Pasteur [Paris] [IP]
Institut Pasteur de la Guyane

Date de publication :

2023-03-21

Mot(s)-clé(s) en anglais :

Species Identification Capillary Electrophoresis ITS2 Length Polymorphism Anopheles French Guiana
Species Identification
Capillary Electrophoresis
ITS2
Length Polymorphism
Anopheles
French Guiana

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

In the Anopheles genus, various mosquito species are able to transmit Plasmodium parasites responsible for malaria, while others are non-vectors. In an effort to better understand the biology of Anopheles species and to ...
Lire la suite >In the Anopheles genus, various mosquito species are able to transmit Plasmodium parasites responsible for malaria, while others are non-vectors. In an effort to better understand the biology of Anopheles species and to quantify transmission risk in an area, the identification of mosquito species collected on the field is an essential but problematic task. Morphological identification requires expertise, well-preserved specimens and high-quality equipment, and it does not allow any subsequent verification when samples are later used in a destructive treatment. Moreover, it involves physical manipulations that are not compatible with experiments requiring fast sampling and processing of specimens, hence species identification is often based on DNA sequencing of reference genes or region such as the Internal Transcribed Spacer 2 (ITS2) region of nuclear ribosomal DNA. Sequencing ITS2 for numerous samples is costly, but the design of species-specific PCR primers is not always possible when local species diversity is high. Here, we introduce a molecular technique of species identification based on precise determination of ITS2 length combined with a simple visual observation, the color of mosquito hindleg tip. DNA extracted from field-collected Anopheles mosquitoes was amplified with universal Anopheles ITS2 primers and analyzed with a capillary electrophoresis device, which precisely determines the size of the fragments. We defined windows of amplicon sizes combined with fifth hind tarsus color, which allow to discriminate the major Anopheles species found in our collections. We validated our parameters via Sanger sequencing of the ITS2 amplicons. This method can be particularly useful in situations with a moderate species diversity, i.e. when the number of local species is too high to define species-specific primers but low enough to avoid individual ITS2 sequencing. This tool will be of interest to evaluate local malaria transmission risk and this approach may further be implemented for other mosquito genera.Lire moins >

Langue :

Anglais

Projet ANR :

Integrative Biology of Emerging Infectious Diseases
Remodelage du microbiote de moustique pour étudier son impact sur la transmission du paludisme
CEnter of the study of Biodiversity in Amazonia

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL