Titre :

Secretory Vesicles Are the Principal Means of SARS-CoV-2 Egress

Auteur(s) :

Eymieux, Sébastien [Auteur]
Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents [MAVIVHe]
Uzbekov, Rustem [Auteur]
Plateforme IBISA de Microscopie Electronique [CHRU de Tours] [PFME-Tours]
Rouillé, Yves [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Blanchard, Emmanuelle [Auteur]
Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents [MAVIVHe]
Hourioux, Christophe [Auteur]
Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents [MAVIVHe]
Dubuisson, Jean [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Belouzard, Sandrine [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Roingeard, Philippe [Auteur]
Morphogénèse et antigénicité du VIH, des Virus des Hépatites et Emergents [MAVIVHe]

Titre de la revue :

Cells

Pagination :

2047

Éditeur :

MDPI

Date de publication :

2021-08

ISSN :

2073-4409

Mot(s)-clé(s) en anglais :

SARS-CoV-2 coronavirus COVID-19 virus egress virus release transmission electron microscopy serial sections
SARS-CoV-2
coronavirus
COVID-19
virus egress
virus release
transmission electron microscopy
serial sections

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

The mechanisms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) egress, similar to those of other coronaviruses, remain poorly understood. The virus buds in intracellular compartments and is therefore thought ...
Lire la suite >The mechanisms of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) egress, similar to those of other coronaviruses, remain poorly understood. The virus buds in intracellular compartments and is therefore thought to be released by the biosynthetic secretory pathway. However, several studies have recently challenged this hypothesis. It has been suggested that coronaviruses, including SARS-CoV-2, use lysosomes for egress. In addition, a focused ion-beam scanning electron microscope (FIB/SEM) study suggested the existence of exit tunnels linking cellular compartments rich in viral particles to the extracellular space resembling those observed for the human immunodeficiency (HIV) in macrophages. Here, we analysed serial sections of Vero cells infected with SARS-CoV-2 by transmission electron microscopy (TEM). We found that SARS-CoV-2 was more likely to exit the cell in small secretory vesicles. Virus trafficking within the cells involves small vesicles, with each generally containing a single virus particle. These vesicles then fuse with the plasma membrane to release the virus into the extracellular space. This work sheds new light on the late stages of the SARS-CoV-2 infectious cycle of potential value for guiding the development of new antiviral strategies.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL