An open microfluidic coculture model of ...
Type de document :
Article dans une revue scientifique
PMID :
URL permanente :
Titre :
An open microfluidic coculture model of fibroblasts and eosinophils to investigate mechanisms of airway inflammation.
Auteur(s) :
Zeng, Yuting [Auteur]
University of Washington [Seattle]
Su, Xiaojing [Auteur]
University of Washington [Seattle]
Takezawa, Meg G [Auteur]
University of Washington [Seattle]
Fichtinger, Paul S [Auteur]
University of Wisconsin-Madison
Lee, Ulri N [Auteur]
University of Wisconsin-Madison
Pippin, Jeffery W [Auteur]
University of Wisconsin-Madison
Shankland, Stuart J [Auteur]
University of Wisconsin-Madison
Lim, Fang Yun [Auteur]
University of Washington [Seattle]
Denlinger, Loren C [Auteur]
University of Wisconsin-Madison
Jarjour, Nizar N [Auteur]
University of Wisconsin-Madison
Mathur, Sameer K [Auteur]
University of Wisconsin-Madison
Sandbo, Nathan [Auteur]
University of Wisconsin-Madison
Berthier, Erwin [Auteur]
University of Washington [Seattle]
Esnault, Stephane [Auteur]
University of Wisconsin-Madison
Bernau, Ksenija [Auteur]
University of Wisconsin-Madison
Theberge, Ashleigh B [Auteur]
University of Washington [Seattle]
University of Washington [Seattle]
Su, Xiaojing [Auteur]
University of Washington [Seattle]
Takezawa, Meg G [Auteur]
University of Washington [Seattle]
Fichtinger, Paul S [Auteur]
University of Wisconsin-Madison
Lee, Ulri N [Auteur]
University of Wisconsin-Madison
Pippin, Jeffery W [Auteur]
University of Wisconsin-Madison
Shankland, Stuart J [Auteur]
University of Wisconsin-Madison
Lim, Fang Yun [Auteur]
University of Washington [Seattle]
Denlinger, Loren C [Auteur]
University of Wisconsin-Madison
Jarjour, Nizar N [Auteur]
University of Wisconsin-Madison
Mathur, Sameer K [Auteur]
University of Wisconsin-Madison
Sandbo, Nathan [Auteur]
University of Wisconsin-Madison
Berthier, Erwin [Auteur]
University of Washington [Seattle]
Esnault, Stephane [Auteur]
University of Wisconsin-Madison
Bernau, Ksenija [Auteur]
University of Wisconsin-Madison
Theberge, Ashleigh B [Auteur]
University of Washington [Seattle]
Titre de la revue :
Frontiers in Bioengineering and Biotechnology
Nom court de la revue :
Front Bioeng Biotechnol
Numéro :
10
Pagination :
993872
Éditeur :
Frontiers
Date de publication :
2022-09-29
ISSN :
2296-4185
Mot(s)-clé(s) en anglais :
cell signaling
coculture
eosinophils
fibroblasts
inflammation
open microfluidics
coculture
eosinophils
fibroblasts
inflammation
open microfluidics
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Interactions between fibroblasts and immune cells play an important role in tissue inflammation. Previous studies have found that eosinophils activated with interleukin-3 (IL-3) degranulate on aggregated immunoglobulin G ...
Lire la suite >Interactions between fibroblasts and immune cells play an important role in tissue inflammation. Previous studies have found that eosinophils activated with interleukin-3 (IL-3) degranulate on aggregated immunoglobulin G (IgG) and release mediators that activate fibroblasts in the lung. However, these studies were done with eosinophil-conditioned media that have the capacity to investigate only one-way signaling from eosinophils to fibroblasts. Here, we demonstrate a coculture model of primary normal human lung fibroblasts (HLFs) and human blood eosinophils from patients with allergy and asthma using an open microfluidic coculture device. In our device, the two types of cells can communicate via two-way soluble factor signaling in the shared media while being physically separated by a half wall. Initially, we assessed the level of eosinophil degranulation by their release of eosinophil-derived neurotoxin (EDN). Next, we analyzed the inflammation-associated genes and soluble factors using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and multiplex immunoassays, respectively. Our results suggest an induction of a proinflammatory fibroblast phenotype of HLFs following the coculture with degranulating eosinophils, validating our previous findings. Additionally, we present a new result that indicate potential impacts of activated HLFs back on eosinophils. This open microfluidic coculture platform provides unique opportunities to investigate the intercellular signaling between the two cell types and their roles in airway inflammation and remodeling.Lire moins >
Lire la suite >Interactions between fibroblasts and immune cells play an important role in tissue inflammation. Previous studies have found that eosinophils activated with interleukin-3 (IL-3) degranulate on aggregated immunoglobulin G (IgG) and release mediators that activate fibroblasts in the lung. However, these studies were done with eosinophil-conditioned media that have the capacity to investigate only one-way signaling from eosinophils to fibroblasts. Here, we demonstrate a coculture model of primary normal human lung fibroblasts (HLFs) and human blood eosinophils from patients with allergy and asthma using an open microfluidic coculture device. In our device, the two types of cells can communicate via two-way soluble factor signaling in the shared media while being physically separated by a half wall. Initially, we assessed the level of eosinophil degranulation by their release of eosinophil-derived neurotoxin (EDN). Next, we analyzed the inflammation-associated genes and soluble factors using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and multiplex immunoassays, respectively. Our results suggest an induction of a proinflammatory fibroblast phenotype of HLFs following the coculture with degranulating eosinophils, validating our previous findings. Additionally, we present a new result that indicate potential impacts of activated HLFs back on eosinophils. This open microfluidic coculture platform provides unique opportunities to investigate the intercellular signaling between the two cell types and their roles in airway inflammation and remodeling.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Établissement(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Date de dépôt :
2023-10-18T11:35:52Z
2023-11-08T10:54:46Z
2023-11-08T10:54:46Z
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