RNA Seq profiling reveals a novel expression ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
RNA Seq profiling reveals a novel expression pattern of TGF-β target genes in human blood eosinophils.
Auteur(s) :
Shen, Zhong-Jian [Auteur]
University of Texas Southwestern Medical Center [Dallas]
Hu, Jie [Auteur]
University of Texas Southwestern Medical Center [Dallas]
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Dozmorov, Igor [Auteur]
University of Texas Southwestern Medical Center [Dallas]
Malter, James S [Auteur]
University of Texas Southwestern Medical Center [Dallas]
University of Texas Southwestern Medical Center [Dallas]
Hu, Jie [Auteur]
University of Texas Southwestern Medical Center [Dallas]
Esnault, Stéphane [Auteur]
University of Wisconsin School of Medicine and Public Health
Dozmorov, Igor [Auteur]
University of Texas Southwestern Medical Center [Dallas]
Malter, James S [Auteur]
University of Texas Southwestern Medical Center [Dallas]
Titre de la revue :
Immunology Letters
Nom court de la revue :
Immunol Lett
Numéro :
167
Pagination :
1-10
Date de publication :
2015-09-01
ISSN :
1879-0542
Mot(s)-clé(s) en anglais :
Asthma
Computational Biology
Eosinophils
Gene Expression Profiling
Gene Expression Regulation
High-Throughput Nucleotide Sequencing
Humans
Molecular Sequence Annotation
Signal Transduction
Transcriptome
Transforming Growth Factor beta
Asthma
Blood
Eosinophils
Gene expression
Human
RNA-Seq
TGF-β
Computational Biology
Eosinophils
Gene Expression Profiling
Gene Expression Regulation
High-Throughput Nucleotide Sequencing
Humans
Molecular Sequence Annotation
Signal Transduction
Transcriptome
Transforming Growth Factor beta
Asthma
Blood
Eosinophils
Gene expression
Human
RNA-Seq
TGF-β
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Despite major advances in our understanding of TGF-β signaling in multiple cell types, little is known about the direct target genes of this pathway in human eosinophils. These cells constitute the major inflammatory ...
Lire la suite >Despite major advances in our understanding of TGF-β signaling in multiple cell types, little is known about the direct target genes of this pathway in human eosinophils. These cells constitute the major inflammatory component present in the sputum and lung of active asthmatics and their numbers correlate well with disease severity. During the transition from acute to chronic asthma, TGF-β levels rise several fold in the lung which drives fibroblasts to produce extracellular matrix (ECM) and participate in airway and parenchymal remodeling. In this report, we use purified blood eosinophils from healthy donors and analyze baseline and TGF-β responsive genes by RNA Seq, and demonstrate that eosinophils (PBE) express 7981 protein-coding genes of which 178 genes are up-regulated and 199 genes are down-regulated by TGF-β. While 18 target genes have been previously associated with asthma and eosinophilic disorders, the vast majority have been implicated in cell death and survival, differentiation, and cellular function. Ingenuity pathway analysis revealed that 126 canonical pathways are activated by TGF-β including iNOS, TREM1, p53, IL-8 and IL-10 signaling. As TGF-β is an important cytokine for eosinophil function and survival, and pulmonary inflammation and fibrosis, our results represent a significant step toward the identification of novel TGF-β responsive genes and provide a potential therapeutic opportunity by selectively targeting relevant genes and pathways.Lire moins >
Lire la suite >Despite major advances in our understanding of TGF-β signaling in multiple cell types, little is known about the direct target genes of this pathway in human eosinophils. These cells constitute the major inflammatory component present in the sputum and lung of active asthmatics and their numbers correlate well with disease severity. During the transition from acute to chronic asthma, TGF-β levels rise several fold in the lung which drives fibroblasts to produce extracellular matrix (ECM) and participate in airway and parenchymal remodeling. In this report, we use purified blood eosinophils from healthy donors and analyze baseline and TGF-β responsive genes by RNA Seq, and demonstrate that eosinophils (PBE) express 7981 protein-coding genes of which 178 genes are up-regulated and 199 genes are down-regulated by TGF-β. While 18 target genes have been previously associated with asthma and eosinophilic disorders, the vast majority have been implicated in cell death and survival, differentiation, and cellular function. Ingenuity pathway analysis revealed that 126 canonical pathways are activated by TGF-β including iNOS, TREM1, p53, IL-8 and IL-10 signaling. As TGF-β is an important cytokine for eosinophil function and survival, and pulmonary inflammation and fibrosis, our results represent a significant step toward the identification of novel TGF-β responsive genes and provide a potential therapeutic opportunity by selectively targeting relevant genes and pathways.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Date de dépôt :
2023-10-27T14:48:44Z
2024-03-16T08:26:42Z
2024-03-16T08:26:42Z
Fichiers
- EOS TGFb Immunol Letters 2015 RNA-seq.pdf
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