s-SHIP Promoter Expression Identifies Mouse ...
Type de document :
Compte-rendu et recension critique d'ouvrage: Autre communication scientifique (congrès sans actes - poster - séminaire...)
Titre :
s-SHIP Promoter Expression Identifies Mouse Mammary Cancer Stem Cells
Auteur(s) :
Tian, Lu [Auteur]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Truong, Marie-José [Auteur]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Lagadec, Chann [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Adriaenssens, Eric [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bouchaert, Emmanuel [Auteur]
Bauderlique-Le Roy, Hélène [Auteur]
Plateforme BioImaging Center Lille - PLBS [BICeL]
Figeac, Martin [Auteur]
Université de Lille
Le Bourhis, Xuefen [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bourette, Roland [Auteur correspondant]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Truong, Marie-José [Auteur]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Lagadec, Chann [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Adriaenssens, Eric [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bouchaert, Emmanuel [Auteur]
Bauderlique-Le Roy, Hélène [Auteur]
Plateforme BioImaging Center Lille - PLBS [BICeL]
Figeac, Martin [Auteur]
Université de Lille
Le Bourhis, Xuefen [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bourette, Roland [Auteur correspondant]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Titre de la revue :
Stem Cell Reports
Pagination :
10-20
Éditeur :
Elsevier
Date de publication :
2019
ISSN :
2213-6711
Mot(s)-clé(s) en anglais :
mammary cancer stem cells
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Cancer
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Résumé en anglais : [en]
During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary ...
Lire la suite >During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary cancer stem cells (CSCs), we generated bitransgenic mice by crossing the C3(1)-SV40 T-antigen transgenic mouse model of breast cancer, and a transgenic mouse (11.5kb-GFP) expressing green fluorescent protein from the s-SHIP promoter. Here we show that in mammary tumors originating in these bitransgenic mice, s-SHIP promoter expression enriches a rare cell population with CSC activity as demonstrated by sphere-forming assays in vitro and limiting dilution transplantation in vivo. These s-SHIP-positive CSCs are characterized by lower expression of Delta-like non-canonical Notch ligand 1 (DLK1), a negative regulator of the Notch pathway. Inactivation of Dlk1 in s-SHIP-negative tumor cells increases their tumorigenic potential, suggesting a role for DLK1 in mammary cancer stemness.Lire moins >
Lire la suite >During normal mammary gland development, s-SHIP promoter expression marks a distinct type of mammary stem cells, at two different stages, puberty and early mid-pregnancy. To determine whether s-SHIP is a marker of mammary cancer stem cells (CSCs), we generated bitransgenic mice by crossing the C3(1)-SV40 T-antigen transgenic mouse model of breast cancer, and a transgenic mouse (11.5kb-GFP) expressing green fluorescent protein from the s-SHIP promoter. Here we show that in mammary tumors originating in these bitransgenic mice, s-SHIP promoter expression enriches a rare cell population with CSC activity as demonstrated by sphere-forming assays in vitro and limiting dilution transplantation in vivo. These s-SHIP-positive CSCs are characterized by lower expression of Delta-like non-canonical Notch ligand 1 (DLK1), a negative regulator of the Notch pathway. Inactivation of Dlk1 in s-SHIP-negative tumor cells increases their tumorigenic potential, suggesting a role for DLK1 in mammary cancer stemness.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
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