Titre :

Adipocyte-induced transdifferentiation of osteoblasts and its potential role in age-related bone loss

Auteur(s) :

Clabaut, Aline [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Grare, Céline [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Rolland-Valognes, Gaëlle [Auteur]
Institut de Recherches SERVIER [IRS]
Letarouilly, Jean-Guillaume [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Bourrier, Chantal [Auteur]
Institut de Recherches SERVIER [IRS]
Andersen, Thomas [Auteur]
Sikjær, Tanja [Auteur]
Aarhus University Hospital
Rejnmark, Lars [Auteur]
Aarhus University Hospital
Ejersted, Charlotte [Auteur]
Pastoureau, Philippe [Auteur]
Institut de Recherches SERVIER [IRS]
Hardouin, Pierre [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Sabatini, Massimo [Auteur]
Broux, Odile [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]

Titre de la revue :

PLOS ONE

Pagination :

e0245014

Éditeur :

Public Library of Science

Date de publication :

2021-01-26

ISSN :

1932-6203

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Our preliminary findings have lead us to propose bone marrow adipocyte secretions as new contributors to bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble ...
Lire la suite >Our preliminary findings have lead us to propose bone marrow adipocyte secretions as new contributors to bone loss. Indeed, using a coculture model based on human bone marrow stromal cells, we previously showed that soluble factors secreted by adipocytes induced the conversion of osteoblasts towards an adipocyte-like phenotype. In this study, microarray gene expression profiling showed profound transcriptomic changes in osteoblasts following coculture and confirmed the enrichment of the adipocyte gene signature. Double immunofluorescence microscopic analyses demonstrated the coexpression of adipogenic and osteoblastic specific markers in individual cells, providing evidence for a transdifferentiation event. At the molecular level, this conversion was associated with upregulated expression levels of reprogramming genes and a decrease in the DNA methylation level. In line with these in vitro results, preliminary immunohistochemical analysis of bone sections revealed adipogenic marker expression in osteoblasts from elderly subjects. Altogether, these data suggest that osteoblast transdifferentiation could contribute to decreased bone mass upon ageing.Lire moins >

Langue :

Anglais

Vulgarisation :

Non

Collections :

• Marrow Adiposity & Bone Lab (MABLab) - ULR 4490

Source :

Harvested from HAL