Titre :

Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages

Auteur(s) :

Manček-Keber, Mateja [Auteur]
Centre of Excelence EN-FIST [Ljubljana]
National Institute of Chemistry [Llubljana] [KI]
Ribić, Rosana [Auteur]
University North [Croatia]
Chain, Fernando [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Sinnaeve, Davy [Auteur]
Biologie Structurale Intégrative [ERL 9002 - INSERM U1167 - BSI]
Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Martins, José [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Jerala, Roman [Auteur]
Centre of Excelence EN-FIST [Ljubljana]
National Institute of Chemistry [Llubljana] [KI]
Tomić, Srđanka [Auteur]
University of Zagreb
Fehér, Krisztina [Auteur correspondant]
University of Debrecen
Hungarian Academy of Sciences [MTA]
Heidelberg Institute for Theoretical Studies [HITS ]

Titre de la revue :

Molecules

Pagination :

3707

Éditeur :

MDPI

Date de publication :

2020-08-14

ISSN :

1420-3049

Mot(s)-clé(s) en anglais :

peptidoglycan
immunostimulation
lipid incapsulation
adamantane
mannose

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Immunologie/Vaccinologie
Sciences du Vivant [q-bio]/Immunologie/Immunité innée

Résumé en anglais : [en]

We report the enhancement of the lipopolysaccharide-induced immune response by adamantane containing peptidoglycan fragments in vitro. The immune stimulation was detected by Il-6 (interleukine 6) and RANTES (regulated on ...
Lire la suite >We report the enhancement of the lipopolysaccharide-induced immune response by adamantane containing peptidoglycan fragments in vitro. The immune stimulation was detected by Il-6 (interleukine 6) and RANTES (regulated on activation, normal T cell expressed and secreted) chemokine expression using cell assays on immortalized mouse bone-marrow derived macrophages. The most active compound was a α-D-mannosyl derivative of an adamantylated tripeptide with L-chirality at the adamantyl group attachment, whereby the mannose moiety assumed to target mannose receptors expressed on macrophage cell surfaces. The immune co-stimulatory effect was also influenced by the configuration of the adamantyl center, revealing the importance of specific molecular recognition event taking place with its receptor. The immunostimulating activities of these compounds were further enhanced upon their incorporation into lipid bilayers, which is likely related to the presence of the adamantyl group that helps anchor the peptidoglycan fragment into lipid nanoparticles. We concluded that the proposed adamantane containing peptidoglycan fragments act as co-stimulatory agents and are also suitable for the preparation of lipid nanoparticle-based delivery of peptidoglycan fragments.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

Développement de stratégies innovantes pour une approche transdisciplinaire de la maladie d'Alzheime

Collections :

• Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167

Source :

Harvested from HAL