Identification of <em>Cryptosporidium ...
Document type :
Compte-rendu et recension critique d'ouvrage
DOI :
Title :
Identification of <em>Cryptosporidium parvum</em> IIa and IId zoonotic subtype families and <em>Cryptosporidium bovis</em> from calves in Algeria
Author(s) :
Sahraoui, Lynda [Auteur]
Ecole Nationale Supérieure Vétérinaire d'Alger [ENSV]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Mammeri, Mohamed [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Thomas, Myriam [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chevillot, Aurélie [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Polack, Bruno [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Vallée, Isabelle [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Follet, Jerome [Auteur]
JUNIA [JUNIA]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Ain-Baaziz, Hacina [Auteur]
Ecole Nationale Supérieure Vétérinaire d'Alger [ENSV]
Adjou, Karim Tarik [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Ecole Nationale Supérieure Vétérinaire d'Alger [ENSV]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Mammeri, Mohamed [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Thomas, Myriam [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chevillot, Aurélie [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Polack, Bruno [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Vallée, Isabelle [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Follet, Jerome [Auteur]
JUNIA [JUNIA]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Ain-Baaziz, Hacina [Auteur]
Ecole Nationale Supérieure Vétérinaire d'Alger [ENSV]
Adjou, Karim Tarik [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Journal title :
Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux
Pages :
1-6
Publication date :
2023-11-15
ISSN :
0035-1865
English keyword(s) :
Cryptosporidium parvum, calves, Algeria
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Cryptosporidiosis is a significant disease in calves caused by the parasitic protist Cryptosporidium. The infection results in severe symptoms such as diarrhea, dehydration, delayed growth, and weight loss, often leading ...
Show more >Cryptosporidiosis is a significant disease in calves caused by the parasitic protist Cryptosporidium. The infection results in severe symptoms such as diarrhea, dehydration, delayed growth, and weight loss, often leading to mortality and economic losses. This study aimed to detect Cryptosporidium spp. in fecal samples from calves in five Algerian provinces. A total of 65 fecal samples from calves were collected from 12 dairy cattle farms in the north-east of Algeria. The presence of the parasites was established by microscopic screening of the oocysts following an immunofluorescence assay (IFA). IFA-positive samples were analyzed by 18S rRNA PCR-RFLP (restriction fragment length polymorphism) to determine the species. Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium oocysts were detected microscopically in 41/65 (63%) samples, of which 26/41 (63.4%) were positive by 18S rRNA PCR-RFLP. Two Cryptosporidium species were detected in 24 samples; C. parvum (20/24) and C. bovis (4/24). C. parvum isolates from IIa and IId zoonotic subtype families were detected: IIaA16G2R1 (9/24), IIdA16G1 (4/24), and IIaA15G2R1 (1/24). Thus, calves are reservoirs of zoonotic C. parvum subtypes and represent a public health concern.Show less >
Show more >Cryptosporidiosis is a significant disease in calves caused by the parasitic protist Cryptosporidium. The infection results in severe symptoms such as diarrhea, dehydration, delayed growth, and weight loss, often leading to mortality and economic losses. This study aimed to detect Cryptosporidium spp. in fecal samples from calves in five Algerian provinces. A total of 65 fecal samples from calves were collected from 12 dairy cattle farms in the north-east of Algeria. The presence of the parasites was established by microscopic screening of the oocysts following an immunofluorescence assay (IFA). IFA-positive samples were analyzed by 18S rRNA PCR-RFLP (restriction fragment length polymorphism) to determine the species. Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium oocysts were detected microscopically in 41/65 (63%) samples, of which 26/41 (63.4%) were positive by 18S rRNA PCR-RFLP. Two Cryptosporidium species were detected in 24 samples; C. parvum (20/24) and C. bovis (4/24). C. parvum isolates from IIa and IId zoonotic subtype families were detected: IIaA16G2R1 (9/24), IIdA16G1 (4/24), and IIaA15G2R1 (1/24). Thus, calves are reservoirs of zoonotic C. parvum subtypes and represent a public health concern.Show less >
Language :
Anglais
Popular science :
Non
Source :
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