Titre :

Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood-Brain Barrier Model Derived from Hematopoietic Stem Cells

Auteur(s) :

Dehouck, Marie-Pierre [Auteur]
Tachikawa, Masanori [Auteur]
Hoshi, Yutaro [Auteur]
Omori, Kotaro [Auteur]
Maurage, Claude-Alain [Auteur]
Service de pathologie [CHU Lille]
Strecker, Guillaume [Auteur]
Dehouck, Lucie [Auteur]
Boucau, Marie-Christine [Auteur]
Uchida, Yasuo [Auteur]
Gosselet, Fabien [Auteur]
Terasaki, Tetsuya [Auteur]
Karamanos, Yannis [Auteur]

Titre de la revue :

Cells

Numéro :

11

Pagination :

3963

Éditeur :

MDPI

Date de publication :

2022-12-24

ISSN :

2073-4409

Mot(s)-clé(s) en anglais :

blood-brain barrier
human in vitro models
human brain-like endothelial cells
brain microvessels
LC-MS
MS-based protein quantification
transporter

Discipline(s) HAL :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]/Neurobiologie
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]

Résumé en anglais : [en]

We previously developed an in vitro model of the human blood–brain barrier (BBB) based on the use of endothelial cells derived from CD34+-hematopoietic stem cells and cultured with brain pericytes. The purpose of the present ...
Lire la suite >We previously developed an in vitro model of the human blood–brain barrier (BBB) based on the use of endothelial cells derived from CD34+-hematopoietic stem cells and cultured with brain pericytes. The purpose of the present study was to provide information on the protein expression levels of the transporters, receptors, tight junction/adherence junction molecules, and transporter-associated molecules of human brain-like endothelial cells (hBLECs). The absolute protein expression levels were determined by liquid chromatography–mass spectrometry-based quantitative targeted absolute proteomics and compared with those from human brain microvessels (hBMVs). The protein levels of CD144, CD147, MRP4, Annexin A6 and caveolin-1 showed more than 3-fold abundance in hBLECs, those of MCT1, Connexin 43, TfR1, and claudin-5 showed less than 3-fold differences, and the protein levels of other drug efflux transporters and nutrient transporters were less represented in hBLECs than in hBMVs. It is noteworthy that BCRP was more expressed than MDR1 in hBLECs, as this was the case for hBMVs. These results suggest that transports mediated by MCT1, TfR1, and claudin-5-related tight junction function reflect the in vivo BBB situation. The present study provided a better characterization of hBLECs and clarified the equivalence of the transport characteristics between in vitro BBB models and in vivo BBB models using LC-MS/MS-based protein quantification.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Autres travaux scientifiques

Date de dépôt :

2024-01-16T00:01:57Z
2025-01-17T13:18:40Z