Titre :

Revisiting the involvement of tau in complex neural network remodeling: analysis of the extracellular neuronal activity in organotypic brain slice co-cultures

Auteur(s) :

Bouillet, Thomas [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Ciba, Manuel [Auteur]
Lourenço Alves, Caroline [Auteur]
Rodrigues, Francisco Aparecido [Auteur]
Thielemann, Christiane [Auteur]
Colin, Morvane [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Buee, Luc [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Halliez, Sophie [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172

Titre de la revue :

Journal of Neural Engineering

Nom court de la revue :

J Neural Eng

Numéro :

19

Pagination :

066026

Éditeur :

IOP Publishing

Date de publication :

2022-12-06

ISSN :

1741-2552

Mot(s)-clé(s) en anglais :

Tau
Neural networks
Hippocampus
Entorhinal cortex
Neuronal activity
Synchrony
Connectivity
Excitation/inhibition ratio

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Objective. Tau ablation has a protective effect in epilepsy due to inhibition of the hyperexcitability/hypersynchrony. Protection may also occur in transgenic models of Alzheimer's disease by reducing the epileptic activity ...
Lire la suite >Objective. Tau ablation has a protective effect in epilepsy due to inhibition of the hyperexcitability/hypersynchrony. Protection may also occur in transgenic models of Alzheimer's disease by reducing the epileptic activity and normalizing the excitation/inhibition imbalance. However, it is difficult to determine the exact functions of tau, because tau knockout (tauKO) brain networks exhibit elusive phenotypes. In this study, we aimed to further explore the physiological role of tau using brain network remodeling. Approach. The effect of tau ablation was investigated in hippocampal-entorhinal slice co-cultures during network remodeling. We recorded the spontaneous extracellular neuronal activity over 2 weeks in single-slice cultures and co-cultures from control and tauKO mice. We compared the burst activity and applied concepts and analytical tools intended for the analysis of the network synchrony and connectivity. Main results. Comparison of the control and tauKO co-cultures revealed that tau ablation had an anti-synchrony effect on the hippocampal-entorhinal two-slice networks at late stages of culture, in line with the literature. Differences were also found between the single-slice and co-culture conditions, which indicated that tau ablation had differential effects at the sub-network scale. For instance, tau ablation was found to have an anti-synchrony effect on the co-cultured hippocampal slices throughout the culture, possibly due to a reduction in the excitation/inhibition ratio. Conversely, tau ablation led to increased synchrony in the entorhinal slices at early stages of the co-culture, possibly due to homogenization of the connectivity distribution. Significance. The new methodology presented here proved useful for investigating the role of tau in the remodeling of complex brain-derived neural networks. The results confirm previous findings and hypotheses concerning the effects of tau ablation on neural networks. Moreover, the results suggest, for the first time, that tau has multifaceted roles that vary in different brain sub-networks.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Lille Neurosciences & Cognition (LilNCog) - U 1172

Équipe(s) de recherche :

Alzheimer et Tauopathies

Date de dépôt :

2024-01-16T00:22:58Z
2025-02-21T11:11:02Z