Titre :

Shielding islets with human amniotic epithelial cells enhances islet engraftment and revascularization in a murine diabetes model.

Auteur(s) :

Lebreton, Fanny [Auteur]
Bellofatto, Kevin [Auteur]
Wassmer, Charles H. [Auteur]
Perez, Lisa [Auteur]
Lavallard, Vanessa [Auteur]
Parnaud, Géraldine [Auteur]
Cottet-Dumoulin, David [Auteur]
Pattou Kerr-Conte, Julie [Auteur]
Recherche translationnelle sur le diabète (RTD) - U1190
Recherche translationnelle sur le diabète (RTD) - U1190
Pattou, Francois [Auteur]
Recherche translationnelle sur le diabète (RTD) - U1190
Bosco, Domenico [Auteur]
Othenin-Girard, Véronique [Auteur]
Martinez De Tejada, Begona [Auteur]
Berishvili, Ekaterine [Auteur]

Titre de la revue :

American Journal of Transplantation

Nom court de la revue :

Am. J. Transplant.

Numéro :

20

Pagination :

1551-1561

Date de publication :

2020-06

ISSN :

1600-6143

Mot(s)-clé(s) en anglais :

basic (laboratory) research
science
diabetes
type 1
islet transplantation
islets of Langerhans
regenerative medicine
stem cells
tissue
organ engineering
translational research
science

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Hypoxia is a major cause of considerable islet loss during the early posttransplant period. Here, we investigate whether shielding islets with human amniotic epithelial cells (hAECs), which possess anti-inflammatory and ...
Lire la suite >Hypoxia is a major cause of considerable islet loss during the early posttransplant period. Here, we investigate whether shielding islets with human amniotic epithelial cells (hAECs), which possess anti-inflammatory and regenerative properties, improves islet engraftment and survival. Shielded islets were generated on agarose microwells by mixing rat islets (RIs) or human islets (HI) and hAECs (100 hAECs/IEQ). Islet secretory function and viability were assessed after culture in hypoxia (1% O2) or normoxia (21% O2) in vitro. In vivo function was evaluated after transplant under the kidney capsule of diabetic immunodeficient mice. Graft morphology and vascularization were evaluated by immunohistochemistry. Both shielded RIs and HIs show higher viability and increased glucose-stimulated insulin secretion after exposure to hypoxia in vitro compared with control islets. Transplant of shielded islets results in considerably earlier normoglycemia and vascularization, an enhanced glucose tolerance, and a higher β cell mass. Our results show that hAECs have a clear cytoprotective effect against hypoxic damages in vitro. This strategy improves β cell mass engraftment and islet revascularization, leading to an improved capacity of islets to reverse hyperglycemia, and could be rapidly applicable in the clinical situation seeing that the modification to HIs are minor.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Recherche translationnelle sur le diabète (RTD) - U1190

Date de dépôt :

2024-01-19T23:34:47Z
2024-10-30T13:12:55Z