Titre :

Heterogeneity of human pancreatic islet isolation around Europe: results of a survey study

Auteur(s) :

Nano, Rita [Auteur]
IRCCS San Raffaele Scientific Institute [Milan, Italie]
Pattou Kerr-Conte, Julie [Auteur]
Recherche translationnelle sur le diabète (RTD) - U1190
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Scholz, Hanne [Auteur]
Oslo University Hospital [Oslo]
Engelse, Marten [Auteur]
Leiden University Medical Center [LUMC]
Karlsson, Marie [Auteur]
Uppsala University Hospital
Saudek, Frantisek [Auteur]
Institute for Clinical and Experimental Medicine [IKEM]
Bosco, Domenico [Auteur]
Hôpitaux Universitaires de Genève [HUG]
Antonioli, Barbara [Auteur]
Bertuzzi, Federico [Auteur]
Johnson, Paul R.V. [Auteur]
University of Oxford
Ludwing, Barbara [Auteur]
University Hospital Carl Gustav Carus [Dresden, Germany]
Ling, Zhidong [Auteur]
Vrije Universiteit Brussel [Bruxelles] [VUB]
De Paep, Diedert L. [Auteur]
Vrije Universiteit Brussel [Bruxelles] [VUB]
Keymeulen, Bart [Auteur]
Vrije Universiteit Brussel [Bruxelles] [VUB]
Pattou, Francois [Auteur]
Recherche translationnelle sur le diabète (RTD) - U1190
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Berney, Thierry [Auteur]
Hôpitaux Universitaires de Genève [HUG]
Korsgren, Olle [Auteur]
Uppsala University Hospital
De Koning, Eelco [Auteur]
Leiden University Medical Center [LUMC]
Piemonti, Lorenzo [Auteur]
Universita Vita Salute San Raffaele = Vita-Salute San Raffaele University [Milan, Italie] [UniSR]
IRCCS San Raffaele Scientific Institute [Milan, Italie]

Titre de la revue :

Transplantation

Nom court de la revue :

Transplantation

Numéro :

104

Pagination :

190-196

Éditeur :

Lippincott, Williams & Wilkins

Date de publication :

2020-01

ISSN :

1534-6080

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Background. Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in ...
Lire la suite >Background. Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation processes within European countries. Methods. A web-based questionnaire about critical steps, including donor selection, pancreas processing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, and release criteria of the product, was completed by isolation facilities participating at the Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan. Results. Eleven islet isolation facilities completed the questionnaire. The facilities reported 445 and 53 islet isolations per year over the last 3 years from deceased organ donors and pancreatectomized patients, respectively. This activity resulted in 120 and 40 infusions per year in allograft and autograft recipients, respectively. Differences among facilities emerged in donor selection (age, cold ischemia time, intensive care unit length, amylase concentration), pancreas procurement, isolation procedures (brand and concentration of collagenase, additive, maximum acceptable digestion time), quality evaluation, and release criteria for transplantation (glucose-stimulated insulin secretion tests, islet numbers, and purity). Moreover, even when a high concordance about the relevance of one parameter was evident, thresholds for the acceptance were different among facilities. Conclusions. The result highlighted the presence of a heterogeneity in the islet cell product process and product release criteria.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Recherche translationnelle sur le diabète (RTD) - U1190

Date de dépôt :

2024-01-19T23:47:21Z
2024-10-04T09:10:57Z