Title :

Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease

Author(s) :

Duan, Yi [Auteur]
Llorente, Cristina [Auteur]
Lang, Sonja [Auteur]
Brandl, Katharina [Auteur]
Chu, Huikuan [Auteur]
Jiang, Lu [Auteur]
White, Richard C. [Auteur]
Clarke, Thomas H. [Auteur]
Nguyen, Kevin [Auteur]
Torralba, Manolito G. [Auteur]
Shao, Yan [Auteur]
Liu, Jinyuan [Auteur]
Hernandez-Morales, Adriana [Auteur]
Lessor, Lauren [Auteur]
Rahman, Imran R. [Auteur]
Miyamoto, Yukiko [Auteur]
Ly, Melissa [Auteur]
Gao, Bei [Auteur]
Sun, Weizhong [Auteur]
Kiesel, Roman [Auteur]
Hutmacher, Felix [Auteur]
Lee, Suhan [Auteur]
Ventura-Cots, Meritxell [Auteur]
Bosques-Padilla, Francisco [Auteur]
Verna, Elizabeth C. [Auteur]
Abraldes, Juan G. [Auteur]
Brown, Robert S. [Auteur]
Vargas, Victor [Auteur]
Altamirano, Jose [Auteur]
Caballeria, Juan [Auteur]
Shawcross, Debbie [Auteur]
Ho, Samuel B. [Auteur]
Louvet, Alexandre [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lucey, Michael R. [Auteur]
Mathurin, Philippe [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Garcia-Tsao, Guadalupe [Auteur]
Bataller, Ramon [Auteur]
Tu, Xin M. [Auteur]
Eckmann, Lars [Auteur]
Van Der Donk, Wilfred A. [Auteur]
Young, Ry [Auteur]
Lawley, Trevor D. [Auteur]
Starkel, Peter [Auteur]
Université Catholique de Louvain = Catholic University of Louvain [UCL]
Pride, David [Auteur]
Fouts, Derrick E. [Auteur]
Schnabl, Bernd [Auteur]

Journal title :

Nature

Abbreviated title :

Nature

Volume number :

575

Pages :

505–511

Publication date :

2019-11-21

ISSN :

1476-4687

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Chronic liver disease due to alcohol-use disorder contributes markedly to the global burden of disease and mortality1,2,3. Alcoholic hepatitis is a severe and life-threatening form of alcohol-associated liver disease. The ...
Show more >Chronic liver disease due to alcohol-use disorder contributes markedly to the global burden of disease and mortality1,2,3. Alcoholic hepatitis is a severe and life-threatening form of alcohol-associated liver disease. The gut microbiota promotes ethanol-induced liver disease in mice4, but little is known about the microbial factors that are responsible for this process. Here we identify cytolysin—a two-subunit exotoxin that is secreted by Enterococcus faecalis5,6—as a cause of hepatocyte death and liver injury. Compared with non-alcoholic individuals or patients with alcohol-use disorder, patients with alcoholic hepatitis have increased faecal numbers of E. faecalis. The presence of cytolysin-positive (cytolytic) E. faecalis correlated with the severity of liver disease and with mortality in patients with alcoholic hepatitis. Using humanized mice that were colonized with bacteria from the faeces of patients with alcoholic hepatitis, we investigated the therapeutic effects of bacteriophages that target cytolytic E. faecalis. We found that these bacteriophages decrease cytolysin in the liver and abolish ethanol-induced liver disease in humanized mice. Our findings link cytolytic E. faecalis with more severe clinical outcomes and increased mortality in patients with alcoholic hepatitis. We show that bacteriophages can specifically target cytolytic E. faecalis, which provides a method for precisely editing the intestinal microbiota. A clinical trial with a larger cohort is required to validate the relevance of our findings in humans, and to test whether this therapeutic approach is effective for patients with alcoholic hepatitis.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2024-01-30T10:27:18Z
2024-04-30T08:46:35Z