Evaluation and Modelling of the Performance ...
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Article dans une revue scientifique: Article original
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Title :
Evaluation and Modelling of the Performance of an Automated SARS-CoV-2 Antigen Assay According to Sample Type, Target Population and Epidemic Trends
Author(s) :
Yin, Nicolas [Auteur]
Université libre de Bruxelles [ULB]
Debuysschere, Cyril [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Daubie, Valery [Auteur]
Université libre de Bruxelles [ULB]
Hildebrand, Marc [Auteur]
Université libre de Bruxelles [ULB]
Martin, Charlotte [Auteur]
Curac, Sonja [Auteur]
Ponthieux, Fanny [Auteur]
Université libre de Bruxelles [ULB]
Payen, Marie-Christine [Auteur]
Université libre de Bruxelles [ULB]
Vandenberg, Olivier [Auteur]
Université libre de Bruxelles [ULB]
Hallin, Marie [Auteur]
Université libre de Bruxelles [ULB]
Université libre de Bruxelles [ULB]
Debuysschere, Cyril [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Daubie, Valery [Auteur]
Université libre de Bruxelles [ULB]
Hildebrand, Marc [Auteur]
Université libre de Bruxelles [ULB]
Martin, Charlotte [Auteur]
Curac, Sonja [Auteur]
Ponthieux, Fanny [Auteur]
Université libre de Bruxelles [ULB]
Payen, Marie-Christine [Auteur]
Université libre de Bruxelles [ULB]
Vandenberg, Olivier [Auteur]
Université libre de Bruxelles [ULB]
Hallin, Marie [Auteur]
Université libre de Bruxelles [ULB]
Journal title :
Diagnostics
Abbreviated title :
Diagnostics
Volume number :
12
Pages :
447
Publication date :
2023-05-02
ISSN :
2075-4418
English keyword(s) :
SARS-CoV-2
COVID-19
model
diagnostic
test
assay
COVID-19
model
diagnostic
test
assay
HAL domain(s) :
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
English abstract : [en]
The Lumipulse® G SARS-CoV-2 Ag assay performance was evaluated on prospectively collected saliva and nasopharyngeal swabs (NPS) of recently ill in- and outpatients and according to the estimated viral load. Performances ...
Show more >The Lumipulse® G SARS-CoV-2 Ag assay performance was evaluated on prospectively collected saliva and nasopharyngeal swabs (NPS) of recently ill in- and outpatients and according to the estimated viral load. Performances were calculated using RT-PCR positive NPS from patients with symptoms ≤ 7 days and RT-PCR negative NPS as gold standard. In addition, non-selected positive NPS were analyzed to assess the performances on various viral loads. This assay yielded a sensitivity of 93.1% on NPS and 71.4% on saliva for recently ill patients. For NPS with a viral load > 103 RNA copies/mL, sensitivity was 96.4%. A model established on our daily routine showed fluctuations of the performances depending on the epidemic trends but an overall good negative predictive value. Lumipulse® G SARS-CoV-2 assay yielded good performance for an automated antigen detection assay on NPS. Using it for the detection of recently ill patients or to screen high-risk patients could be an interesting alternative to the more expensive RT-PCR.Show less >
Show more >The Lumipulse® G SARS-CoV-2 Ag assay performance was evaluated on prospectively collected saliva and nasopharyngeal swabs (NPS) of recently ill in- and outpatients and according to the estimated viral load. Performances were calculated using RT-PCR positive NPS from patients with symptoms ≤ 7 days and RT-PCR negative NPS as gold standard. In addition, non-selected positive NPS were analyzed to assess the performances on various viral loads. This assay yielded a sensitivity of 93.1% on NPS and 71.4% on saliva for recently ill patients. For NPS with a viral load > 103 RNA copies/mL, sensitivity was 96.4%. A model established on our daily routine showed fluctuations of the performances depending on the epidemic trends but an overall good negative predictive value. Lumipulse® G SARS-CoV-2 assay yielded good performance for an automated antigen detection assay on NPS. Using it for the detection of recently ill patients or to screen high-risk patients could be an interesting alternative to the more expensive RT-PCR.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CHU Lille
CHU Lille
Collections :
Submission date :
2024-02-02T22:05:33Z
2024-02-13T09:01:29Z
2024-02-13T09:01:29Z
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