Is orange carotenoid protein photoactivation a&nbsp;single-photon process?

Niziński, Stanisław; Schlichting, Ilme; Colletier, Jacques-Philippe; Kirilovsky, Diana; Burdzinski, Gotard; Sliwa, Michel

Type de document :

Article dans une revue scientifique: Article original

DOI :

10.1016/j.bpr.2022.100072

PMID :

36425326

URL permanente :

http://hdl.handle.net/20.500.12210/109483

Titre :

Is orange carotenoid protein photoactivation a single-photon process?

Auteur(s) :

Niziński, Stanisław [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Schlichting, Ilme [Auteur]
Max Planck Institute for Medical Research [Heidelberg]
Colletier, Jacques-Philippe [Auteur]
Institut de biologie structurale [IBS - UMR 5075]
Kirilovsky, Diana [Auteur]
Institut de Biologie Intégrative de la Cellule [I2BC]
Burdzinski, Gotard [Auteur]
Sliwa, Michel [Auteur]

Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Titre de la revue :

Biophys Rep (N Y)

Nom court de la revue :

Biophys Rep (N Y)

Numéro :

Pagination :

100072

Date de publication :

2022-11-26

ISSN :

2667-0747

Discipline(s) HAL :

Chimie/Chimie théorique et/ou physique

Résumé en anglais : [en]

In all published photoactivation mechanisms of orange carotenoid protein (OCP), absorption of a single photon by the orange dark state starts a cascade of red-shifted OCP ground-state intermediates that subsequently decay ...
Lire la suite >In all published photoactivation mechanisms of orange carotenoid protein (OCP), absorption of a single photon by the orange dark state starts a cascade of red-shifted OCP ground-state intermediates that subsequently decay within hundreds of milliseconds, resulting in the formation of the final red form OCPR, which is the biologically active form that plays a key role in cyanobacteria photoprotection. A major challenge in deducing the photoactivation mechanism is to create a uniform description explaining both single-pulse excitation experiments, involving single-photon absorption, and continuous light irradiation experiments, where the red-shifted OCP intermediate species may undergo re-excitation. We thus investigated photoactivation of Synechocystis OCP using stationary irradiation light with a biologically relevant photon flux density coupled with nanosecond laser pulse excitation. The kinetics of photoactivation upon continuous and nanosecond pulse irradiation light show that the OCPR formation quantum yield increases with photon flux density; thus, a simple single-photon model cannot describe the data recorded for OCP in vitro. The results strongly suggest a consecutive absorption of two photons involving a red intermediate with ≈100 millisecond lifetime. This intermediate is required in the photoactivation mechanism and formation of the red active form OCPR.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS

Collections :

Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516

Équipe(s) de recherche :

Dynamics, Nanoscopy & Chemometrics (DyNaChem)

Date de dépôt :

2024-02-28T22:16:49Z
2024-03-13T08:57:24Z
2024-03-13T08:59:55Z

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Ce document est diffusé sous licence Attribution-NonCommercial-NoDerivs 3.0 United States

Historique des modifications

Numéro de version	Lien	Date de modification
3	20.500.12210/109483*	2024-03-13T08:59:44Z
2	20.500.12210/109483.2	2024-03-13T08:50:17Z
1	20.500.12210/109483.1	2024-02-28T22:16:49Z

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