Title :

Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock

Author(s) :

Azkanaz, Maria [Auteur]
Rodríguez López, Aida [Auteur]
de Boer, Bauke [Auteur]
Huiting, Wouter [Auteur]
Angrand, Pierre-Olivier [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Université de Lille
Vellenga, Edo [Auteur]
Kampinga, Harm [Auteur]
Bergink, Steven [Auteur]
Martens, Joost Ha [Auteur]
Schuringa, Jan Jacob [Auteur]
van den Boom, Vincent [Auteur]

Journal title :

eLife

Publisher :

eLife Sciences Publication

Publication date :

2019-06-14

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Maintenance of epigenetic modifiers is of utmost importance to preserve the epigenome and consequently appropriate cellular functioning. Here, we analyzed Polycomb group protein (PcG) complex integrity in response to heat ...
Show more >Maintenance of epigenetic modifiers is of utmost importance to preserve the epigenome and consequently appropriate cellular functioning. Here, we analyzed Polycomb group protein (PcG) complex integrity in response to heat shock (HS). Upon HS, various Polycomb Repressive Complex (PRC)1 and PRC2 subunits, including CBX proteins, but also other chromatin regulators, are found to accumulate in the nucleolus. In parallel, binding of PRC1/2 to target genes is strongly reduced, coinciding with a dramatic loss of H2AK119ub and H3K27me3 marks. Nucleolar-accumulated CBX proteins are immobile, but remarkably both CBX protein accumulation and loss of PRC1/2 epigenetic marks are reversible. This post-heat shock recovery of pan-nuclear CBX protein localization and reinstallation of epigenetic marks is HSP70 dependent. Our findings demonstrate that the nucleolus is an essential protein quality control center, which is indispensable for recovery of epigenetic regulators and maintenance of the epigenome after heat shock.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL