Characterization of mouse muc6 and evidence ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Characterization of mouse muc6 and evidence of conservation of the gel-forming mucin gene cluster between human and mouse.
Auteur(s) :
Titre de la revue :
Genomics
Nom court de la revue :
Genomics
Numéro :
81
Pagination :
433-6
Date de publication :
2003-04-01
ISSN :
0888-7543
Mot(s)-clé(s) en anglais :
Animals
Base Sequence
Blotting
Southern
Chromosome Mapping
DNA Primers
Gene Expression Profiling
Humans
Mice
Molecular Sequence Data
Mucin-6
Mucins
Reverse Transcriptase Polymerase Chain Reaction
Base Sequence
Blotting
Southern
Chromosome Mapping
DNA Primers
Gene Expression Profiling
Humans
Mice
Molecular Sequence Data
Mucin-6
Mucins
Reverse Transcriptase Polymerase Chain Reaction
Résumé en anglais : [en]
Using degenerate primers designed from conserved cysteine-rich domains of gel-forming mucins, we cloned two new mouse mucin cDNAs. Blast searching showed that they belong to the same new gene assigned to chromosome 7 band ...
Lire la suite >Using degenerate primers designed from conserved cysteine-rich domains of gel-forming mucins, we cloned two new mouse mucin cDNAs. Blast searching showed that they belong to the same new gene assigned to chromosome 7 band F5. This gene is clustered with the three secreted large gel-forming mucins Muc2, Muc5ac, and Muc5b in a region that exhibits synteny with human chromosome 11p15. Computer analysis and sequence alignments with mucin genes predict that the new gene is composed of 33 exons and spans 30 kb from the initiation ATG codon to the Stop codon. Sequence similarities, domain organization of the deduced peptide, and expression analysis allow us to conclude that this newly cloned mouse gene is Muc6, i.e., the mouse ortholog of human MUC6. Like those of their human homologs, the genomic order and arrangement of the four mucins within the cluster of mucin genes are conserved.Lire moins >
Lire la suite >Using degenerate primers designed from conserved cysteine-rich domains of gel-forming mucins, we cloned two new mouse mucin cDNAs. Blast searching showed that they belong to the same new gene assigned to chromosome 7 band F5. This gene is clustered with the three secreted large gel-forming mucins Muc2, Muc5ac, and Muc5b in a region that exhibits synteny with human chromosome 11p15. Computer analysis and sequence alignments with mucin genes predict that the new gene is composed of 33 exons and spans 30 kb from the initiation ATG codon to the Stop codon. Sequence similarities, domain organization of the deduced peptide, and expression analysis allow us to conclude that this newly cloned mouse gene is Muc6, i.e., the mouse ortholog of human MUC6. Like those of their human homologs, the genomic order and arrangement of the four mucins within the cluster of mucin genes are conserved.Lire moins >
Audience :
Non spécifiée
Date de dépôt :
2019-07-09T09:09:36Z
Fichiers
- 2003Genomics.pdf
- Non spécifié
- Accès confidentiel
- Accéder au document