Title :

Residence time and uptake of porous and cationic maltodextrin-based nanoparticles in the nasal mucosa: Comparison with anionic and cationic nanoparticles.

Author(s) :

Le, Minh-Quan [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Carpentier, Rodolphe [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lantier, Isabelle [Auteur]
Université de Tours [UT]
Ducournau, Celine [Auteur]

Dimier-Poisson, Isabelle [Auteur]
Université de Tours [UT]
Betbeder, Didier [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

International Journal of Pharmaceutics

Abbreviated title :

Int J Pharm

Volume number :

550

Pages :

316-324

Publication date :

2018-10-25

ISSN :

1873-3476

English keyword(s) :

Mucosa
Nanoparticles
Nasal
Vaccine

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems. These nanoparticles need to strongly interact with mucosa cells to deliver their payload. However, only a few ...
Show more >Different types of biodegradable nanoparticles (NP) have been studied as nasal mucosa cell delivery systems. These nanoparticles need to strongly interact with mucosa cells to deliver their payload. However, only a few simultaneous comparisons have been made and it is therefore difficult to determine the best candidate. Here we compared 5 types of nanoparticles with different surface charge (anionic or cationic) and various inner compositions as potential vectors: cationic and anionic liposomes, cationic and anionic PLGA (Poly Lactic co-Glycolic Acid) NP and porous and cationic maltodextrin NP (cationic surface with an anionic lipid core: NPL). We first quantified their nasal residence time after nasal administration in mice using in vivo live imaging and NPL showed the longest residence time. In vitro endocytosis on mucosal cells (airway epithelial cells, macrophages and dendritic cells) using labeled nanoparticles were performed by flow cytometry and confocal microscopy. Among the 5 nanoparticles, NPL were taken up to the greatest extent by the 3 different cell lines and the endocytosis mechanisms were characterized. Taken together, we observed that the nanoparticles' cationic surface charge is insufficient to improve mucosal residence time and cellular uptake and that the NPL are the best candidates to interact with airway mucosal cells.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-10-22T08:09:58Z
2024-03-01T10:00:10Z