Title :

Comparison of two commercial quantitative PCR assays and correlation with the first WHO International Standard for human CMV

Author(s) :

Engelmann, Ilka [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Alidjinou, Enagnon Kazali [Auteur]
Laboratoire de virologie - ULR 3610
Lazrek, Mouna [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Ogiez, Judith [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pouillaude, Jean-Marie [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Chazard, Emmanuel [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Dewilde, Anny [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Hober, Didier [Auteur]
215293|||Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie] (VALID)

Journal title :

Diagnostic Microbiology and Infectious Disease

Abbreviated title :

Diagn. Microbiol. Infect. Dis.

Volume number :

91

Pages :

27-33

Publisher :

Elsevier

Publication date :

2018-01-07

ISSN :

1879-0070

English keyword(s) :

Viral load
CMV
Molecular testing
WHO standard
PCR

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard). Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP ...
Show more >Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard). Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP or MagNA Pure LC System followed by the kPCR PLX™ CMV DNA (kPCR) or the CMV R-gene™ assay (R-gene), respectively; 139 clinical specimens were tested. Both assays correlated well with the standard (R2 > 0.96) and a matrix effect was observed. Quantitative results correlated reasonably between both assays for whole blood (R2 = 0.79) and well for other specimen types (R2 = 0.93). Quantification differences were within one log10 of the averaged log10 results for 25/27 blood specimens and for 32/33 other specimens. Calibration to the standard did not increase this percentage. In conclusion, results of both assays showed reasonable correlation with each other and good correlation with the standard. Calibration to the standard did not improve comparability of quantitative results.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Université de Lille

Collections :

• Laboratoire de virologie - ULR 3610
• METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694

Submission date :

2020-02-11T09:07:36Z
2024-02-23T10:09:52Z