The role of epicuticular waxes on foliar ...
Document type :
Article dans une revue scientifique
Permalink :
Title :
The role of epicuticular waxes on foliar metal transfer and phytotoxicity in edible vegetables: case of Brassica oleracea species exposed to manufactured particles
Author(s) :
Dappe, Vincent [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Dumez, Sylvain [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Bernard, Fabien [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Hanoune, Benjamin [Auteur]
Physicochimie des Processus de Combustion et de l’Atmosphère - UMR 8522 [PC2A]
Cuny, Damien [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Dumat, Camille [Auteur]
Institut National Polytechnique (Toulouse) [Toulouse INP]
Centre d'Etude et de Recherche Travail Organisation Pouvoir [CERTOP]
Sobanska, Sophie [Auteur]
1292|||Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Institut des Sciences Moléculaires [ISM]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Dumez, Sylvain [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Bernard, Fabien [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Hanoune, Benjamin [Auteur]
Physicochimie des Processus de Combustion et de l’Atmosphère - UMR 8522 [PC2A]
Cuny, Damien [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Dumat, Camille [Auteur]
Institut National Polytechnique (Toulouse) [Toulouse INP]
Centre d'Etude et de Recherche Travail Organisation Pouvoir [CERTOP]
Sobanska, Sophie [Auteur]
1292|||Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Institut des Sciences Moléculaires [ISM]
Journal title :
Environmental Science and Pollution Research
Abbreviated title :
Environ Sci Pollut Res
Volume number :
26
Pages :
20092-20106
Publisher :
Springer Verlag
Publication date :
2018-09-27
ISSN :
0944-1344
English keyword(s) :
Brassica oleracea
Metallic particles
Foliar transfer
Epicuticular waxes
Phytotoxicity
DNA damage Gene expression
Metallic particles
Foliar transfer
Epicuticular waxes
Phytotoxicity
DNA damage Gene expression
HAL domain(s) :
Physique [physics]/Physique [physics]/Chimie-Physique [physics.chem-ph]
Sciences de l'ingénieur [physics]
Chimie/Chimie théorique et/ou physique
Physique [physics]/Physique [physics]/Chimie-Physique [physics.chem-ph]
Sciences de l'ingénieur [physics]
Chimie/Chimie théorique et/ou physique
Physique [physics]/Physique [physics]/Chimie-Physique [physics.chem-ph]
English abstract : [en]
The rapid industrialization and urbanization of intra- and peri-urban areas at the world scale are responsible for the degradation of the quality of edible crops, because of their contamination with airborne pollutants. ...
Show more >The rapid industrialization and urbanization of intra- and peri-urban areas at the world scale are responsible for the degradation of the quality of edible crops, because of their contamination with airborne pollutants. Their consumption could lead to serious health risks. In this work, we aim to investigate the phytotoxicity induced by foliar transfer of atmospheric particles of industrial/urban origin. Leaves of cabbage plants (Brassica oleracea var. Prover) were contaminated with metal-rich particles (PbSO4 CuO and CdO) of micrometer size. A trichloroacetic acid (TCA) treatment was used to inhibit the synthesis of the epicuticular waxes in order to investigate their protective role against metallic particles toxicity. Besides the location of the particles on/in the leaves by microscopic techniques, photosynthetic activity measurements, genotoxicity assessment, and quantification of the gene expression have been studied for several durations of exposure (5, 10, and 15 days). The results show that the depletion of epicuticular waxes has a limited effect on the particle penetration in the leaf tissues. The stomatal openings appear to be the main pathway of particles entry inside the leaf tissues, as demonstrated by the overexpression of the BolC.CHLI1 gene. The effects of particles on the photosynthetic activity are limited, considering only the photosynthetic Fv/Fm parameter. The genotoxic effects were significant for the contaminated TCA-treated plants, especially after 10 days of exposure. Still, the cabbage plants are able to implement repair mechanisms quickly, and to thwart the physiological effects induced by the particles. Finally, the foliar contamination by metallic particles induces no serious damage to DNA, as observed by monitoring the BolC.OGG1 gene.Show less >
Show more >The rapid industrialization and urbanization of intra- and peri-urban areas at the world scale are responsible for the degradation of the quality of edible crops, because of their contamination with airborne pollutants. Their consumption could lead to serious health risks. In this work, we aim to investigate the phytotoxicity induced by foliar transfer of atmospheric particles of industrial/urban origin. Leaves of cabbage plants (Brassica oleracea var. Prover) were contaminated with metal-rich particles (PbSO4 CuO and CdO) of micrometer size. A trichloroacetic acid (TCA) treatment was used to inhibit the synthesis of the epicuticular waxes in order to investigate their protective role against metallic particles toxicity. Besides the location of the particles on/in the leaves by microscopic techniques, photosynthetic activity measurements, genotoxicity assessment, and quantification of the gene expression have been studied for several durations of exposure (5, 10, and 15 days). The results show that the depletion of epicuticular waxes has a limited effect on the particle penetration in the leaf tissues. The stomatal openings appear to be the main pathway of particles entry inside the leaf tissues, as demonstrated by the overexpression of the BolC.CHLI1 gene. The effects of particles on the photosynthetic activity are limited, considering only the photosynthetic Fv/Fm parameter. The genotoxic effects were significant for the contaminated TCA-treated plants, especially after 10 days of exposure. Still, the cabbage plants are able to implement repair mechanisms quickly, and to thwart the physiological effects induced by the particles. Finally, the foliar contamination by metallic particles induces no serious damage to DNA, as observed by monitoring the BolC.OGG1 gene.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Other project(s) or funding source(s) :
Région Hauts-de-France
IRENI
FEDER (n°42525)
IRENI
FEDER (n°42525)
Collections :
Research team(s) :
PhysicoChimie de l'Atmosphère (PCA)
Submission date :
2018-12-07T10:54:49Z
2018-12-14T17:30:07Z
2018-12-19T10:29:09Z
2019-04-29T14:25:45Z
2022-10-03T10:30:56Z
2024-01-31T09:34:23Z
2018-12-14T17:30:07Z
2018-12-19T10:29:09Z
2019-04-29T14:25:45Z
2022-10-03T10:30:56Z
2024-01-31T09:34:23Z
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