First identification of Cryptosporidium ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
Titre :
First identification of Cryptosporidium parvum zoonotic subtype IIaA15G2R1 in diarrheal lambs in France
Auteur(s) :
Mammeri, Mohamed [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Cartou, Lara [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chevillot, Aurélie [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Thomas, Myriam [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Julien, Christine [Auteur]
Vallée, Isabelle [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Polack, Bruno [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Follet, Jerome [Auteur]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Yncréa Hauts-de-France
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Adjou, Karim Tarik [Auteur correspondant]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Cartou, Lara [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chevillot, Aurélie [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Thomas, Myriam [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Julien, Christine [Auteur]
Vallée, Isabelle [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Polack, Bruno [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Follet, Jerome [Auteur]

Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Yncréa Hauts-de-France
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Adjou, Karim Tarik [Auteur correspondant]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Titre de la revue :
Veterinary Parasitology : Regional Studies and Reports
Éditeur :
Elsevier
Date de publication :
2019-12
ISSN :
2405-9390
Mot(s)-clé(s) en anglais :
Cryptosporidium
Lamb
Sheep
Zoonosis
France
Lamb
Sheep
Zoonosis
France
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animale
Résumé en anglais : [en]
To date, no information is available about the presence of Cryptosporidium spp. in French sheep, nor their potential role as zoonotic reservoirs. A total of 23 fecal samples were collected from diarrheic lambs (< 11 days ...
Lire la suite >To date, no information is available about the presence of Cryptosporidium spp. in French sheep, nor their potential role as zoonotic reservoirs. A total of 23 fecal samples were collected from diarrheic lambs (< 11 days old) from seven randomly selected farms. Cryptosporidium-oocysts were detected microscopically with Direct Immunofluorescence Assays (DFA) in 23/23 (100%) of fecal samples. PCR-RFLP of the 18S rRNA gene was used to determine species in all samples, and only Cryptosporidium parvum was identified. Isolates were subtyped by sequencing the 60 kDa glycoprotein (gp60) gene. Two zoonotic subtypes within the IIa subtype family were identified, including IIaA15G2R1 (22/23) and IIaA16G3R1 (1/23). This study reports for the first time the identification and genotyping of zoonotic C. parvum subtypes from lambs in France. Sheep could thus play an important role as potential reservoirs for this zoonotic protist.Lire moins >
Lire la suite >To date, no information is available about the presence of Cryptosporidium spp. in French sheep, nor their potential role as zoonotic reservoirs. A total of 23 fecal samples were collected from diarrheic lambs (< 11 days old) from seven randomly selected farms. Cryptosporidium-oocysts were detected microscopically with Direct Immunofluorescence Assays (DFA) in 23/23 (100%) of fecal samples. PCR-RFLP of the 18S rRNA gene was used to determine species in all samples, and only Cryptosporidium parvum was identified. Isolates were subtyped by sequencing the 60 kDa glycoprotein (gp60) gene. Two zoonotic subtypes within the IIa subtype family were identified, including IIaA15G2R1 (22/23) and IIaA16G3R1 (1/23). This study reports for the first time the identification and genotyping of zoonotic C. parvum subtypes from lambs in France. Sheep could thus play an important role as potential reservoirs for this zoonotic protist.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Source :
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