Title :

Humoral Immunity Links Candida albicans Infection and Celiac Disease

Author(s) :

Corouge, Marion [Auteur]
Service des Maladies de l'Appareil Digestif et de la Nutrition [CHRU Lille]
Loridant, Severine [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Fradin, Chantal [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Salleron, Julia [Auteur]
Service de Biostatistiques [CHRU Lille]
Damiens, Sebastien [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Moragues Maria, Dolores [Auteur]
Universidad del País Vasco [Espainia] / Euskal Herriko Unibertsitatea [España] = University of the Basque Country [Spain] = Université du pays basque [Espagne] [UPV / EHU]
Souplet, Vianney [Auteur]
Jouault, Thierry [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Robert, Raymond [Auteur]
Groupe d'Étude des Interactions Hôte-Pathogène [GEIHP]
Dubucquoi, Sylvain [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Sendid, Boualem [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Colombel, Jean-Frederic [Auteur]
Service des Maladies de l'Appareil Digestif et de la Nutrition [CHRU Lille]
Poulain, Daniel [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

PLoS One

Abbreviated title :

PLoS One

Volume number :

10

Publication date :

2015-03-20

ISSN :

1932-6203

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Objective The protein Hwp1, expressed on the pathogenic phase of Candida albicans, presents sequence analogy with the gluten protein gliadin and is also a substrate for transglutaminase. This had led to the suggestion ...
Show more >Objective The protein Hwp1, expressed on the pathogenic phase of Candida albicans, presents sequence analogy with the gluten protein gliadin and is also a substrate for transglutaminase. This had led to the suggestion that C. albicans infection (CI) may be a triggering factor for Celiac disease (CeD) onset. We investigated cross-immune reactivity between CeD and CI. Methods Serum IgG levels against recombinant Hwp1 and serological markers of CeD were measured in 87 CeD patients, 41 CI patients, and 98 healthy controls (HC). IgA and IgG were also measured in 20 individuals from each of these groups using microchips sensitized with 38 peptides designed from the N-terminal of Hwp1. Results CI and CeD patients had higher levels of anti-Hwp1 (p=0.0005 and p=0.004) and anti-gliadin (p=0.002 and p=0.0009) antibodies than HC but there was no significant difference between CeD and CI patients. CeD and CI patients had higher levels of anti-transglutaminase IgA than HC (p=0.0001 and p=0.0039). During CI, the increase in anti-Hwp1 paralleled the increase in anti-gliadin antibodies. Microchip analysis showed that CeD patients were more reactive against some Hwp1 peptides than CI patients, and that some deamidated peptides were more reactive than their native analogs. Binding of IgG from CeD patients to Hwp1 peptides was inhibited by γIII gliadin peptides. Conclusions Humoral cross-reactivity between Hwp1 and gliadin was observed during CeD and CI. Increased reactivity to Hwp1 deamidated peptide suggests that transglutaminase is involved in this interplay. These results support the hypothesis that CI may trigger CeD onset in genetically-susceptible individuals.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Inserm
Université de Lille
CHU Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Research team(s) :

Inflammatory digestive disease : pathophysiology and therapeutic targets developement
Fungal associated invasive and inflammatory diseases
Immunity, inflammation and fibrsis in auto and allo-reactivity
Nutritional modulation of inflammation and infection
IBD and environnemental factors : epidemiology and functional analyses

Submission date :

2019-03-01T14:18:29Z
2022-07-06T10:25:36Z