Titre :

Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring

Auteur(s) :

Shaik, Faruk Azam [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Université de Lille
Lewuillon, Clara [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Université de Lille
Guillemette, Aurélie [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Université de Lille
Ahmadian, Bahram [Auteur]
JUNIA [JUNIA]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Brinster, Carine [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Université de Lille
Quesnel, Bruno [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Université de Lille
Collard, Dominique [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Touil, Yasmine [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Université de Lille
Lemonnier, Loïc [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Tarhan, Mehmet-Cagatay [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
JUNIA [JUNIA]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]

Titre de la revue :

Lab on a Chip

Pagination :

908-920

Éditeur :

Royal Society of Chemistry

Date de publication :

2022

ISSN :

1473-0197

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Immunologie
Sciences du Vivant [q-bio]/Biologie cellulaire
Sciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique

Résumé en anglais : [en]

Analyzing cell–cell interaction is essential to investigate how immune cells function. Elegant designs have been demonstrated to study lymphocytes and their interaction partners. However, these devices have been targeting ...
Lire la suite >Analyzing cell–cell interaction is essential to investigate how immune cells function. Elegant designs have been demonstrated to study lymphocytes and their interaction partners. However, these devices have been targeting cells of similar dimensions. T lymphocytes are smaller, more deformable, and more sensitive to pressure than many cells. This work aims to fill the gap of a method for pairing cells with different dimensions. The developed method uses hydrodynamic flow focusing in the z-direction for on-site modulation of effective channel height to capture smaller cells as single cells. Due to immune cells' sensitivity to pressure, the proposed method provides a stable system without any change in flow conditions at the analysis area throughout experiments. Paired live cells have their activities analyzed with calcium imaging at the immunological synapse formed under a controlled environment. The method is demonstrated with primary human T lymphocytes, acute myeloid leukemia (AML) cell lines, and primary AML blasts.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL