Comparative super-resolution mapping of ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Comparative super-resolution mapping of basal feet reveals a modular but distinct architecture in primary and motile cilia
Auteur(s) :
Nguyen, Quynh P. H. [Auteur]
University of Toronto
Liu, Zhen [Auteur]
University of Toronto
Albulescu, Alexandra [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Ouyang, Hong [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Zlock, Lorna [Auteur]
California Northstate University [CNSU]
Coyaud, Etienne-Marie [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Laurent, Estelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Finkbeiner, Walter [Auteur]
California Northstate University [CNSU]
Moraes, Theo J. [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Raught, Brian [Auteur]
University of Toronto
Mennella, Vito [Auteur]
University of Toronto
University of Toronto
Liu, Zhen [Auteur]
University of Toronto
Albulescu, Alexandra [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Ouyang, Hong [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Zlock, Lorna [Auteur]
California Northstate University [CNSU]
Coyaud, Etienne-Marie [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Laurent, Estelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Finkbeiner, Walter [Auteur]
California Northstate University [CNSU]
Moraes, Theo J. [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Raught, Brian [Auteur]
University of Toronto
Mennella, Vito [Auteur]
University of Toronto
Titre de la revue :
Developmental cell
Nom court de la revue :
Dev. Cell
Numéro :
55
Pagination :
209-223.E7
Éditeur :
Elsevier
Date de publication :
2020-10-26
ISSN :
1534-5807
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
In situ molecular architecture analysis of organelles and protein assemblies is essential to understanding the role of individual components and their cellular function, and to engineering new molecular functionalities. ...
Lire la suite >In situ molecular architecture analysis of organelles and protein assemblies is essential to understanding the role of individual components and their cellular function, and to engineering new molecular functionalities. Through a super-resolution-driven approach, here we characterize the organization of the ciliary basal foot, an appendage of basal bodies whose main role is to provide a point of anchoring to the microtubule cytoskeleton. Quantitative image analysis shows that the basal foot is organized into three main regions linked by elongated coiled-coil proteins, revealing a conserved modular architecture in primary and motile cilia, but showing distinct features reflecting its specialized functions. Using domain-specific BioID proximity labeling and super-resolution imaging, we identify CEP112 as a basal foot protein and other candidate components of this assembly, aiding future investigations on the role of basal foot across different cilia systems.Lire moins >
Lire la suite >In situ molecular architecture analysis of organelles and protein assemblies is essential to understanding the role of individual components and their cellular function, and to engineering new molecular functionalities. Through a super-resolution-driven approach, here we characterize the organization of the ciliary basal foot, an appendage of basal bodies whose main role is to provide a point of anchoring to the microtubule cytoskeleton. Quantitative image analysis shows that the basal foot is organized into three main regions linked by elongated coiled-coil proteins, revealing a conserved modular architecture in primary and motile cilia, but showing distinct features reflecting its specialized functions. Using domain-specific BioID proximity labeling and super-resolution imaging, we identify CEP112 as a basal foot protein and other candidate components of this assembly, aiding future investigations on the role of basal foot across different cilia systems.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
INSERM
Université de Lille
Université de Lille
Date de dépôt :
2022-06-15T13:58:01Z
2023-03-08T08:30:10Z
2024-02-14T07:18:33Z
2024-05-22T08:41:28Z
2023-03-08T08:30:10Z
2024-02-14T07:18:33Z
2024-05-22T08:41:28Z
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