Titre :

A proximity-dependent biotinylation map of a human cell

Auteur(s) :

Go, Christopher D. [Auteur]
Knight, James D. R. [Auteur]
Rajasekharan, Archita [Auteur]
Rathod, Bhavisha [Auteur]
Hesketh, Geoffrey G. [Auteur]
Abe, Kento T. [Auteur]
Youn, Ji-Young [Auteur]
Samavarchi-Tehrani, Payman [Auteur]
Zhang, Hui [Auteur]
Zhu, Lucie Y. [Auteur]
Popiel, Evelyn [Auteur]
Lambert, Jean-Philippe [Auteur]
Coyaud, Etienne-Marie [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Cheung, Sally W. T. [Auteur]
Rajendran, Dushyandi [Auteur]
Wong, Cassandra J. [Auteur]
Antonicka, Hana [Auteur]
Pelletier, Laurence [Auteur]
Palazzo, Alexander F. [Auteur]
Shoubridge, Eric A. [Auteur]
Raught, Brian [Auteur]
Gingras, Anne-Claude [Auteur]

Titre de la revue :

Nature

Nom court de la revue :

Nature

Numéro :

595

Pagination :

120-124

Date de publication :

2021-06-02

ISSN :

0028-0836

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy(1) and biochemical fractionation coupled with mass ...
Lire la suite >Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy(1) and biochemical fractionation coupled with mass spectrometry(2-4) have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of cellular compartments in living cells(5-7). Here we present a BioID-based map of a human cell on the basis of 192 subcellular markers, and define the intracellular locations of 4,145 unique proteins in HEK293 cells. Our localization predictions exceed the specificity of previous approaches, and enabled the discovery of proteins at the interface between the mitochondrial outer membrane and the endoplasmic reticulum that are crucial for mitochondrial homeostasis. On the basis of this dataset, we created humancellmap.org as a community resource that provides online tools for localization analysis of user BioID data, and demonstrate how this resource can be used to understand BioID results better.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

INSERM
Université de Lille

Collections :

• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Date de dépôt :

2022-06-15T13:58:16Z
2023-04-05T09:20:23Z