Carbonic anhydrase xii expression is ...
Type de document :
Article dans une revue scientifique: Article original
DOI :
PMID :
URL permanente :
Titre :
Carbonic anhydrase xii expression is modulated during epithelial mesenchymal transition and regulated through protein kinase c signaling
Auteur(s) :
Vergara, Daniele [Auteur]
University of Salento [Lecce]
Ravaioli, Sara [Auteur]
Unit of Melanoma Medical Oncology [Fondazione IRCCS Istituto Nazionale Tumori, Milan]
Fonzi, Eugenio [Auteur]
Adamo, Loredaria [Auteur]
Damato, Marina [Auteur]
Bravaccini, Sara [Auteur]
Pirini, Francesca [Auteur]
Gaballo, Antonio [Auteur]
Barbano, Raffaela [Auteur]
Pasculli, Barbara [Auteur]
Franck, Julien [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
FOURNIER, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Maffia, Michele [Auteur]
University of Salento [Lecce]
Ravaioli, Sara [Auteur]
Unit of Melanoma Medical Oncology [Fondazione IRCCS Istituto Nazionale Tumori, Milan]
Fonzi, Eugenio [Auteur]
Adamo, Loredaria [Auteur]
Damato, Marina [Auteur]
Bravaccini, Sara [Auteur]
Pirini, Francesca [Auteur]
Gaballo, Antonio [Auteur]
Barbano, Raffaela [Auteur]
Pasculli, Barbara [Auteur]
Franck, Julien [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
FOURNIER, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Maffia, Michele [Auteur]
Titre de la revue :
International Journal of Molecular Sciences
Nom court de la revue :
Int J Mol Sci
Numéro :
21
Pagination :
715
Date de publication :
2020-01-22
ISSN :
1422-0067
Mot(s)-clé(s) :
breast cancer
carbonic anhydrase
epithelial mesenchymal transition
proteomics
PKC
transport metabolon
metabolism
carbonic anhydrase
epithelial mesenchymal transition
proteomics
PKC
transport metabolon
metabolism
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Members of the carbonic anhydrase family are functionally involved in the regulation of intracellular and extracellular pH in physiological and pathological conditions. Their expression is finely regulated to maintain a ...
Lire la suite >Members of the carbonic anhydrase family are functionally involved in the regulation of intracellular and extracellular pH in physiological and pathological conditions. Their expression is finely regulated to maintain a strict control on cellular homeostasis, and it is dependent on the activation of extracellular and intracellular signaling pathways. Combining RNA sequencing (RNA-seq), NanoString, and bioinformatics data, we demonstrated that the expression of carbonic anhydrase 12 (CAXII) is significantly different in luminal and triple negative breast cancer (BC) models and patients, and is associated with the activation of an epithelial mesenchymal transition (EMT) program. In BC models, the phorbol ester 12-myristate 13-acetate (PMA)-mediated activation of protein kinase C (PKC) induced a down-regulation of CAXII with a concomitant modulation of other members of the transport metabolon, including CAIX and the sodium bicarbonate cotransporter 3 (NBCn1). This is associated with a remodeling of tumor glycolytic metabolism induced after PKC activation. Overall, this analysis highlights the dynamic nature of transport metabolom and identifies signaling pathways finely regulating this plasticity.Lire moins >
Lire la suite >Members of the carbonic anhydrase family are functionally involved in the regulation of intracellular and extracellular pH in physiological and pathological conditions. Their expression is finely regulated to maintain a strict control on cellular homeostasis, and it is dependent on the activation of extracellular and intracellular signaling pathways. Combining RNA sequencing (RNA-seq), NanoString, and bioinformatics data, we demonstrated that the expression of carbonic anhydrase 12 (CAXII) is significantly different in luminal and triple negative breast cancer (BC) models and patients, and is associated with the activation of an epithelial mesenchymal transition (EMT) program. In BC models, the phorbol ester 12-myristate 13-acetate (PMA)-mediated activation of protein kinase C (PKC) induced a down-regulation of CAXII with a concomitant modulation of other members of the transport metabolon, including CAIX and the sodium bicarbonate cotransporter 3 (NBCn1). This is associated with a remodeling of tumor glycolytic metabolism induced after PKC activation. Overall, this analysis highlights the dynamic nature of transport metabolom and identifies signaling pathways finely regulating this plasticity.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
INSERM
Université de Lille
Université de Lille
Date de dépôt :
2022-06-15T13:59:25Z
2023-01-11T11:14:40Z
2023-01-11T11:14:40Z
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